Quantitative proteomic analysis of neuronal tissue obtained from patients and animal models of epilepsy

• Grant number: 16/19484-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2016
• End date: October 31, 2020
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Iscia Teresinha Lopes Cendes
• Grantee: Barbara Henning Silva
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/07559-3 - BRAINN - The Brazilian Institute of Neuroscience and Neurotechnology, AP.CEPID
• Associated scholarship(s): 19/12333-0 - A statistical method for detection of copy number variation using next-generation sequencing data, BE.EP.PD

Abstract

Quantifying and comparing the protein profile between healthy and diseased organisms can reveal potentially causing molecular mechanisms of disease. Techniques for identification and quantification of proteins in biological tissues such as mass spectrometry have evolved rapidly and therefore has been produced datasets increasingly larger and more complex. The main objective of the project e'develop and apply proteomic analysis tools in animal models of tissue and surgical material obtained from patients with epilepsy. Specifically we aim to: i) conduct comparative analysis of protein expression to identify and annotate proteins whose expression is altered in affected individuals compared to healthy subjects; ii) provide publicly tools developed during the project execution, through open initiatives such as Biocondutor Project. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CANTO, AMANDA M.; MATOS, ALEXANDRE H. B.; GODOI, ALEXANDRE B.; VIEIRA, ANDRE S.; AOYAMA, BEATRIZ B.; ROCHA, CRISTIANE S.; HENNING, BARBARA; CARVALHO, BENILTON S.; PASCOAL, VINICIUS D. B.; VEIGA, DIOGO F. T.; et al. Multi-omics analysis suggests enhanced epileptogenesis in theCornu Ammonis3 of the pilocarpine model of mesial temporal lobe epilepsy. HIPPOCAMPUS, v. 31, n. 2, p. 122-139, FEB 2021. (16/19484-6, 19/04335-3, 13/08635-5, 13/07559-3, 19/00213-0, 15/12960-4)

CANTO, AMANDA M.; GODOI, ALEXANDRE B.; MATOS, ALEXANDRE H. B.; GERALDIS, JAQUELINE C.; ROGERIO, FABIO; ALVIM, MARINA K. M.; YASUDA, CLARISSA L.; GHIZONI, ENRICO; TEDESCHI, HELDER; VEIGA, DIOGO F. T.; et al. Benchmarking the proteomic profile of animal models of mesial temporal epilepsy. ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY, v. 9, n. 4, p. 14-pg., 2022-03-03. (16/19484-6, 13/07559-3, 19/00213-0, 13/07467-1, 15/12960-4)

CANTO, AMANDA M.; VIEIRA, ANDRE S.; MATOS, ALEXANDRE H. B.; CARVALHO, BENILTON S.; HENNING, BARBARA; NORWOOD, BRAXTON A.; BAUER, SEBASTIAN; ROSENOW, FELIX; GILIOLI, ROVILSON; CENDES, FERNANDO; et al. Laser microdissection-based microproteomics of the hippocampus of a rat epilepsy model reveals regional differences in protein abundances. SCIENTIFIC REPORTS, v. 10, n. 1, MAR 10 2020. (13/07559-3, 13/08635-5, 15/12960-4, 16/19484-6)