| Grant number: | 16/17092-3 |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| Start date: | November 01, 2016 |
| End date: | January 31, 2019 |
| Field of knowledge: | Biological Sciences - Botany - Pant Physiology |
| Agreement: | Federal Ministry of Education and Research, Germany |
| Principal Investigator: | Lázaro Eustaquio Pereira Peres |
| Grantee: | Maísa de Siqueira Pinto |
| Host Institution: | Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil |
| Associated research grant: | 15/50220-2 - Enhancing salt tolerance in tomato crops for advancing sustainable agriculture and food production, AP.TEM |
Abstract Soil salinity is an important issue to crop production in the world, especially due to the increasing global climate changes and the practice of irrigation. As effect of salt stress, the crops exhibit slower growth rates and problems in the reproductive development affecting the yield. The CBL-CIPK signaling system has been identified as a fundamental plant regulatory signaling network that governs stress tolerance and ion transport. In unpublished work, the group of Prof. Dr. Jörg Kudla from Westfälische Wilhelms-Universität Münster identified the Ca2+ sensor-kinase module CBL1/9-CIPK11 from Arabidopsis as essential for activating RbohF. The protein RbohF is a NADPH oxidase that was found to be essential for Casparian strip formation in roots and for establishing salt tolerance in soil. The model tomato plant cultivar Micro-Tom (MT), which has a small size and short life cycle (Campos et al., 2010) have been successfully used in studies involving the production of transgenic plants for physiological and genetic analysis. The use of this cultivar will allow the analysis of a large number of plants and transgenic lines. This proposal aims to genetically dissect the contribution of the identified CBLs, CIPKs, and RbohF to transpiration dependent salt tolerance (TDST) in tomato using the model tomato cultivar Micro-Tom. We will develop loss-of-function and overexpression transgenic lines. The evaluation of these transgenic lines will contribute to better understand the transpiration-dependent salinity tolerance in tomato. (AU) | |
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