Evaluation of immunomodulatory potential of probiotics in periodontal disease

• Grant number: 16/14687-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2016
• End date: September 30, 2019
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Marinella Holzhausen Caldeira
• Grantee: Emmanuel Albuquerque de Souza
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 15/18273-9 - New strategies for the control of periodontitis, AP.TEM
• Associated scholarship(s): 17/25260-6 - THE ACTION OF MARESIN 1 (MAR1) ON FIBROGENESIS AND OSTEOGENIC ACTIVITY OF HUMAN PERIODONTAL LIGAMENT CELLS, BE.EP.DR

Abstract

The use of probiotics, microorganisms safe for human consumption and with large beneficial effects, has shown promise in treating periodontal disease (PD). However the mechanisms by which these agents can act in PD are still unclear. This project will evaluate the effect probiotics application on immune response of gingival epithelial cells challenged with Porphyromonas gingivalis and in the control of experimental PD. The effect of probiotic bacteria of the genus Bifidobacterium sp. and Lactobacillus sp. will be determined in a co-culture test of gingival epithelial cells OBA-9 challenged with P. gingivalis strains, W83 and ATCC 33277, followed by analysis of the ability of adhesion and invasion, wound healing and viability of gingival epithelial cells. Strains or combinations of probiotics that show the most promising results in these preliminary analyzes will be evaluated for their ability to interfere with the OBA-9 cell apoptosis process in co-culture with P. gingivalis and for their ability to modulate the immune response in these cells by flow cytometric analysis, gene expression (RT-PCR), Western blotting and quantitation of beta-defensins and cytokines by ELISA. After these assessments, the effect of probiotic strain or association most promising to control the PD will be determined using a experimental periodontitis model in mice induced by P. gingivalis. In this in vivo model will evaluate the action of probiotics in the development and treatment of PD by the analysis of alveolar bone loss and the characterization of Th1, Th2, Th17 and Treg cells subpopulations present in periodontal tissues and involved in the immune response in PD.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMADO, PAMELA PONTES PENAS; KAWAMOTO, DIONE; ALBUQUERQUE-SOUZA, EMMANUEL; FRANCO, DIEGO CASTILLO; SARAIVA, LUCIANA; CASARIN, RENATO CORREA VIANA; HORLIANA, ANNA CAROLINA RATTO TEMPESTINI; MAYER, MARCIA PINTO ALVES. Oral and Fecal Microbiome in Molar-Incisor Pattern Periodontitis. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, OCT 8 2020. (15/00259-0, 16/14687-6, 16/13159-6, 15/18273-9)

SHIMABUKURO, NATALI; CATARUCI, AMALIA C. DE S.; ISHIKAWA, KARIN H.; OLIVEIRA, BRUNA E. DE; KAWAMOTO, DIONE; ANDO-SUGUIMOTO, ELLEN S.; ALBUQUERQUE-SOUZA, EMMANUEL; NICOLI, JACQUES R.; FERREIRA, CAROLINE M.; DE LIMA, JEAN; et al. Bifidobacterium Strains Present Distinct Effects on the Control of Alveolar Bone Loss in a Periodontitis Experimental Model. FRONTIERS IN PHARMACOLOGY, v. 12, SEP 24 2021. (17/22345-0, 15/18273-9, 16/13156-7, 18/25171-6, 16/14687-6, 17/16377-7, 16/13159-6)

ALBUQUERQUE-SOUZA, EMMANUEL; ISHIKAWA, KARIN HITOMI; AMADO, PAMELA PENAS; NICOLI, JACQUES ROBERT; HOLZHAUSEN, MARINELLA; MAYER, MARCIA P. A.. Probiotics improve re-epithelialization of scratches infected by Porphyromonas gingivalis through up-regulating CXCL8-CXCR1/CXCR2 axis. Anaerobe, v. 72, DEC 2021. (16/13156-7, 15/18273-9, 16/14687-6)

ALBUQUERQUE-SOUZA, EMMANUEL; BALZARINI, DANILO; ANDO-SUGUIMOTO, ELLEN S.; ISHIKAWA, KARIN H.; SIMIONATO, MARIA R. L.; HOLZHAUSEN, MARINELLA; MAYER, MARCIA P. A.. Probiotics alter the immune response of gingival epithelial cells challenged by Porphyromonas gingivalis. JOURNAL OF PERIODONTAL RESEARCH, v. 54, n. 2, p. 115-127, APR 2019. (15/18273-9, 16/14687-6)

BUENO, MANUELA R.; ISHIKAWA, KARIN H.; ALMEIDA-SANTOS, GISLANE; ANDO-SUGUIMOTO, ELLEN S.; SHIMABUKURO, NATALI; KAWAMOTO, DIONE; MAYER, MARCIA P. A.. Lactobacilli Attenuate the Effect of Aggregatibacter actinomycetemcomitans Infection in Gingival Epithelial Cells. FRONTIERS IN MICROBIOLOGY, v. 13, p. 11-pg., 2022-05-04. (16/13159-6, 17/16377-7, 16/13156-7, 16/14687-6, 15/18273-9, 17/22345-0)

ISHIKAWA, KARIN HITOMI; MITA, DANIELA; KAWAMOTO, DIONE; NICOLI, JACQUES ROBERT; ALBUQUERQUE-SOUZA, EMMANUEL; LORENZETTI SIMIONATO, MARIA REGINA; ALVES MAYER, MARCIA PINTO. Probiotics alter biofilm formation and the transcription ofPorphyromonas gingivalisvirulence-associated genes. JOURNAL OF ORAL MICROBIOLOGY, v. 12, n. 1, JAN 1 2020. (16/13156-7, 15/18273-9, 16/14687-6, 16/13159-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, Emmanuel Albuquerque de. Insights from in vitro studies with probiotics and specialized pro-resolving mediators into new strategies in the control of periodontitis. 2019. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.