"Characterization of transcription factors that have the basic domain" zipper "leucine (bZIP) ATF1 in Aspergillus fumigatus"

• Grant number: 16/21392-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2017
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Gustavo Henrique Goldman
• Grantee: Lilian Pereira Silva
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Aspergillus fumigatus is an opportunistic fungus that mainly affects immunocompromised patients. The main syndrome caused by A. fumigatus is invasive pulmonary aspergillosis, which occurs by inhalation of spores. Since immunocompromised patients do not have an active immune defense system, these spores settle in the alveolar Broncos germinating and producing hyphae. Different metabolic pathways are studied to understand the resistance mechanisms of the immune system defenses and virulence of A. fumigatus. The MAPK-HOG1 routes are highlighted in this study because they are related to phosphorylation and activation of transcription factors of the family "zipper" of ATF1 leucine. The ATF1 has aroused interest because of its role in orthologous as Schizosaccharomyces. pombe, a transcriptional activator of genes that respond to osmotic and oxidative stress. Therefore, this study aims to understand and characterize phenotypically and biochemically the four transcription factors (AtfA, AtfB, AtfC and AtfD) family "zipper" ATF1 of leucine in A. fumigatus. We also intend to verify their importance in the process of virulence through the study of two animal models, Galleria mellonela and neutropenic mice.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KNOWLES, SONJA L.; MEAD, MATTHEW E.; SILVA, LILIAN PEREIRA; RAJA, HUZEFA A.; STEENWYK, JACOB L.; GOLDMAN, GUSTAVO H.; OBERLIES, NICHOLAS H.; ROKAS, ANTONIS. Gliotoxin, a Known Virulence Factor in the Major Human Pathogen Aspergillus fumigatus, Is Also Biosynthesized by Its Nonpathogenic Relative Aspergillus fischeri. MBIO, v. 11, n. 1, JAN-FEB 2020. (16/21392-2, 16/07870-9)

ZHAO, SHU; MARTIN-VICENTE, ADELA; COLABARDINI, ANA CRISTINA; SILVA, LILIAN PEREIRA; RINKER, DAVID C.; FORTWENDEL, JARROD R.; GOLDMAN, GUSTAVO HENRIQUE; GIBBONS, JOHN G.. Genomic and Molecular Identification of Genes Contributing to the Caspofungin Paradoxical Effect in Aspergillus fumigatus. MICROBIOLOGY SPECTRUM, v. N/A, p. 18-pg., 2022-09-12. (21/04977-5, 16/21392-2, 17/07536-4)

SILVA, LILIAN PEREIRA; HORTA, MARIA AUGUSTA CRIVELENTE; GOLDMAN, GUSTAVO HENRIQUE. Genetic Interactions Between Aspergillus fumigatus Basic Leucine Zipper (bZIP) Transcription Factors AtfA, AtfB, AtfC, and AtfD. FRONTIERS IN FUNGAL BIOLOGY, v. 2, p. 15-pg., 2021-02-11. (16/21392-2, 16/07870-9)

VALERO, CLARA; COLABARDINI, ANA CRISTINA; DE CASTRO, PATRICIA ALVES; SILVA, LILIAN PEREIRA; RIES, LAURE NICOLAS ANNICK; PARDESHI, LAKHANSING; WANG, FANG; ROCHA, MARINA CAMPOS; MALAVAZI, IRAN; SILVA, ROBERTO NASCIMENTO; et al. Aspergillus Fumigatus ZnfA, a Novel Zinc Finger Transcription Factor Involved in Calcium Metabolism and Caspofungin Tolerance. FRONTIERS IN FUNGAL BIOLOGY, v. 2, p. 16-pg., 2021-08-10. (16/21392-2, 17/07536-4, 16/12948-7, 16/07870-9, 18/00315-5, 17/19694-3, 18/00715-3, 17/14159-2)

DE CASTRO, PATRICIA ALVES; COLABARDINI, ANA CRISTINA; MANFIOLLI, ADRIANA OLIVEIRA; CHIARATTO, JESSICA; SILVA, LILIAN PEREIRA; MATTOS, ELICIANE CEVOLANI; PALMISANO, GIUSEPPE; ALMEIDA, FAUSTO; PERSINOTI, GABRIELA FELIX; ANNICK RIES, LAURE NICOLAS; et al. Aspergillus fumigatus calcium-responsive transcription factors regulate cell wall architecture promoting stress tolerance, virulence and caspofungin resistance. PLOS GENETICS, v. 15, n. 12, DEC 2019. (17/07536-4, 16/21392-2, 14/06863-3, 16/12948-7, 16/07870-9, 16/22062-6, 14/04783-2, 17/19288-5)

SILVA, LILIAN PEREIRA; FRAWLEY, DEAN; DE ASSIS, LEANDRO JOSE; TIERNEY, CIARA; FLEMING, ALASTAIR B.; BAYRAM, OZGUR; GOLDMAN, GUSTAVO HENRIQUE. Putative Membrane Receptors Contribute to Activation and Efficient Signaling of Mitogen-Activated Protein Kinase Cascades during Adaptation of Aspergillus fumigatus to Different Stressors and Carbon Sources. MSPHERE, v. 5, n. 5, SEP-OCT 2020. (16/07870-9, 14/00789-6, 16/21392-2)

DA COSTA FILHO, AILTON PEREIRA; PEREIRA BRANCINI, GUILHERME THOMAZ; DE CASTRO, PATRICIA ALVES; VALERO, CLARA; FERREIRA FILHO, JAIRE ALVES; SILVA, LILIAN PEREIRA; ROCHA, MARINA CAMPOS; MALAVAZI, IRAN; DE MORAES PONTES, JOAO GUILHERME; FILL, TAICIA; et al. Aspergillus fumigatus G-Protein Coupled Receptors GprM and GprJ Are Important for the Regulation of the Cell Wall Integrity Pathway, Secondary Metabolite Production, and Virulence. MBIO, v. 11, n. 5, SEP-OCT 2020. (16/07870-9, 18/10962-8, 18/25217-6, 19/11929-7, 17/14159-2, 16/12948-7, 18/00715-3, 19/10727-1, 19/06359-7, 16/21392-2)

DE CASTRO, PATRICIA ALVES; VALERO, CLARA; CHIARATTO, JESSICA; COLABARDINI, ANA CRISTINA; PARDESHI, LAKHANSING; SILVA, LILIAN PEREIRA; ALMEIDA, FAUSTO; ROCHA, MARINA CAMPOS; SILVA, ROBERTO NASCIMENTO; MALAVAZI, IRAN; et al. Novel Biological Functions of the NsdC Transcription Factor in Aspergillus fumigatus. MBIO, v. 12, n. 1, JAN-FEB 2021. (16/07870-9, 18/00715-3, 16/21392-2, 17/07536-4, 16/22062-6, 16/12948-7, 19/10097-8)

VALERO, CLARA; COLABARDINI, ANA CRISTINA; CHIARATTO, JESSICA; PARDESHI, LAKHANSING; DE CASTRO, PATRICIA ALVES; FERREIRA FILHO, JAIRE ALVES; SILVA, LILIAN PEREIRA; ROCHA, MARINA CAMPOS; MALAVAZI, IRAN; COSTA, JONAS HENRIQUE; et al. Aspergillus fumigatus Transcription Factors Involved in the Caspofungin Paradoxical Effect. MBIO, v. 11, n. 3, p. 22-pg., 2020-05-01. (16/21392-2, 17/07536-4, 16/12948-7, 16/07870-9, 16/22062-6, 18/00715-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Lilian Pereira. Characterization of transcription factors containing the basic leucine zipper (bZIP) ATF1 domain in Aspergillus fumigatus. 2021. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.