Study of biocompatibility of chitosan gel associated with glycerol phosphate to repair bone defects induced experimentally in radio of rabbits (Oryctolagus cuniculus)

• Grant number: 16/09224-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2017
• End date: December 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Bruno Watanabe Minto
• Grantee: Camila Potério Borsaro
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Among the many diseases affecting the bone structure, fractures with bone loss have been widely studied. Tissue engineering is an interdisciplinary science which has been developing biomaterial for the regeneration of bone tissue in medicine and veterinary. Among the polymers, there is chitosan, derived from chitin deacetylation process, potentially attractive for many uses due to its non-toxic nature, antibacterial and antioxidant activity, biodegradability, biocompatibility, abundance, with low cost production and environmentally friendly. The objective of this research is to evaluate bone regeneration obtained with the application of chitosan hydrogel associated with phosphate glycerol in bone loss experimentally induced in radio of rabbits. The project will be developed in the research laboratory of the Veterinary Hospital "Governador Laudo Natel" of UNESP, Jaboticabal - SP. It will be used 15 rabbits composing two groups represented by the radios of each animal, one group will be treated with the chitosan hydrogel associated with the phosphate glycerol (biomaterial group - GB) and the other group will receive no treatment (control group - CG). The animals will be evaluated for surgical wound, radiographic examinations, optical densitometry and histological analysis of the bone-implant interface, in the periods 30, 60 and 90 days postoperatively. (AU)