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Effect of Er,Cr:YSGG laser associated to fluoride application in the control of dentin erosion

Grant number: 16/25883-0
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2017
Effective date (End): March 31, 2018
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Taís Scaramucci Forlin
Grantee:Letícia Rigacci Trevisan
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


This study will evaluate the effect of Er,Cr:YSGG laser, associated to fluoride application, in the control of dentin erosion. Bovine incisors will be used in this study, from which 4mm x 4mm x 2mm dentin slabs will be sectioned from their roots. The slabs will be embedded in acrylic resin, flattened and polished. A tape will be then placed on the polished surface of the specimens, leaving a 4mm x 1mm central area exposed to subsequent testing. Half of the specimens will be previously eroded (10min immersion in 1% citric acid solution, pH~2.4) and half would be kept sound. The sound and eroded specimens (n=10 each substrate) will be randomly allocated into the experimental groups, according to the following surface treatments: 1. Negative control (no treatment); 2. APF gel (1,23% F, pH 3.6-3.9); 3. Er,Cr:YSGG laser irradiation (parameter 1: 0.25W, 20Hz, 2.8J/cm2); 4. Er,Cr:YSGG laser irradiation (parameter 2: 0.50W, 20Hz, 5.7 J/cm2); 5. APF gel + Er,Cr:YSGG laser parameter 1 +; 6. APF gel + Er,Cr:YSGG laser parameter 2. After treatments, the specimens will undergo an erosion-remineralization cycling, consisting of 5min immersion in 0.3% citric acid, followed by 60min exposure to artificial saliva. This procedure will be repeated 4 times a day, for 5 days. At the end of cycling, the tapes will be removed, and dentin surface loss (in µm) will be determined with an optical profilometer. Data will be statistically analyzed, considering a significance level of 5%. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BEZERRA, S. J. C.; TREVISAN, L. R.; VIANA, I. E. L.; LOPES, R. M.; PEREIRA, D. L.; ARANHA, A. C. C.; SCARAMUCCI, TAIS. Er,Cr:YSGG laser associated with acidulated phosphate fluoride gel (1.23% F) for prevention and control of dentin erosion progression. Lasers in Medical Science, v. 34, n. 3, p. 449-455, . (16/25883-0)

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