In cases of severe fires, the fire fight using only water has limitations. In these cases, the use of more efficient compounds combined with water becomes necessary, and may include the Aqueous Film Forming Foams (AFFFs). The presence of active principles of AFFFs has been reported in ecosystems worldwide; however, the information on the toxicity of these compounds toaquatic organisms is still scarce. The present study intends to estimate the toxic effects caused by the AFFFs Ageofoam 2133, Cold Fire (Firefreeze Worldwide Inc.), and F-500 Fire Supressing, isolated and when combined with gasoline. The choice for assessing mixtures with gasoline relies on the fact that fire emergencies are often related to this compound. Tests will be carried out considering the toxicity to Daphnia similis. The results obtained with the isolated substances will provide the CE50-48h for each substance, which will further serve to the establishment of the experimental design for testing the mixtures. This analysis will apply different combinations of substances, considering their respective Toxic Units (TUs). The toxicity assessments of the mixtures and the identification of interaction model swill provide information regulating of the use of AFFF and thus contribute to the improvement of management and protection of the environment.
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