Models of binders rapid screening in extracts of natural products for bioconjugation tests and LC-HRMS

• Grant number: 16/24957-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2017
• End date: December 31, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Agreement: GlaxoSmithKline
• Principal Investigator: Arlene Gonçalves Corrêa
• Grantee: Gabriel Mazzi Leme
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 14/50249-8 - Green chemistry: sustainable synthetic methods employing benign solvents, safer reagents, and bio-renewable feedstock, AP.PCPE

Abstract

The identification of substances with biological activity in natural extracts or synthetic collections is a crucial step in the search for new drugs and requires the use of fast and efficient techniques of separation and screening. This fact encourages the development of new methods that facilitate and reduce the time spent on identifying such substances. The isolation of active principles derived from a natural extract can rarely be achieved in one step, thus strategies that integrate screening, separation and characterization are presented as promising tools in the search for new bioactive compounds. Thus, this project proposes the use of ligand fishing technique for the screening of plant extracts in search of bioactive compounds to human cholinesterases, coupled with high performance liquid chromatography and detection by high-resolution mass spectrometry. The species investigated in this work are plants of the Brazilian cerrado, selected based on ethnobotanical and chemotaxonomic knowledge. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, JULIANA M.; LEME, GABRIEL M.; COSTA, EMMANOEL V.; CASS, QUEZIA B.. LC-HRMS and acetylcholinesterase affinity assay as a workflow for profiling alkaloids in Annona salzmannii extract. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1164, FEB 1 2021. (14/50249-8, 16/24957-0)

VANZOLINI, K. L.; SPRENGER, R. DA F.; LEME, G. M.; MORAES, V. R. DE S.; VILELA, A. F. L.; CARDOSO, C. L.; CASS, Q. B.. Acetylcholinesterase affinity-based screening assay on Lippia gracilis Schauer extracts. Journal of Pharmaceutical and Biomedical Analysis, v. 153, p. 232-237, MAY 10 2018. (13/02054-0, 13/01710-1, 16/24957-0, 14/50249-8)

DO AMARAL, BRUNO S.; DA SILVA, FERNANDA B.; LEME, GABRIEL MAZZI; SCHMITZ, LETICIA S. S.; JIMENEZ, PAULA C.; CAMPOS MARTINS, ROBERTO CARLOS; CASS, QUEZIA B.; VALVERDE, ALESSANDRA L.. Integrated analytical workflow for chromatographic profiling and metabolite annotation of a cytotoxic Phorbas amaranthus extract. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1174, JUN 1 2021. (16/24957-0, 14/50249-8)