Cryopreservation: the crystals ice formation and their relation to the cell injuries

• Grant number: 17/13245-2
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: July 31, 2017
• End date: August 31, 2017
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Elizabeth Romagosa
• Grantee: Taís da Silva Lopes
• Supervisor: Michael A Menze
• Host Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Institution abroad: University of Louisville (UofL), United States
• Associated to the scholarship: 14/21215-8 - Cryopreservation of surubim-do-paraíba (Steindachneridion parahybae) oocites, BP.PD

Abstract

The benefits that cryopreservation of animal cells can provide are innumerable and undeniable. However, many difficulties still exist to establish efficient protocols, and in the case of fish oocytes, to guarantee their viability. In this way, studies are needed to deepen the knowledge about cryobiology of animal cells, the process of ice formation, and their impact on the injuries caused to cryopreserved tissues. To this end, the present Research Internship abroad is intended to provide contact with leading technologies used in human cryobiology studies, as well as to seek guidance from a reference team in the area of cryopreservation, to assist in the discussions of the articles being prepared about the Postdoctoral studies (in progress). These are the techniques developed in the PhD Michael A. Menze laboratory, suitable for application to fish oocytes: studies on mitochondrial bioenergy; isothermal calorimetry; cytotoxicity assays based on fluorescence or protein microscopy; and Raman confocal microspectroscopy (CRM). The results of the activities developed will be presented in the form of a report, and scientific articles will be completed and submitted for publication and dissemination of the results, with the help of the discussions elaborated during the internship.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES, TAIS DA SILVA; ROMAGOSA, ELIZABETH. OOCYTES OF THE ENDANGERED GRAY CATFISH (SURUBIM-DOPARAIBA) SUBMITTED TO DIFFERENT CONSERVATION PROTOCOLS. CRYOLETTERS, v. 41, n. 6, p. 323-329, NOV 2020. (17/13245-2, 14/21215-8)

LOPES, TAIS DA SILVA; SANCHES, EDUARDO ANTONIO; CANEPPELE, DANILO; SILVEIRA, MARIANA MOLICA; ROMAGOSA, ELIZABETH. Chilling sensitivity of Steindachneridion parahybae (Siluriformes: Pimelodidae) oocytes in different cryoprotectants. VETERINARY AND ANIMAL SCIENCE, v. 7, p. 5-pg., 2019-06-01. (14/21215-8, 17/13245-2)