DNA methylation analysis in transcriptional regulation comprehension of relevant genes in gastric carcinogesis

• Grant number: 17/06227-8
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): July 01, 2017
• Effective date (End): December 31, 2017
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Marilia de Arruda Cardoso Smith
• Grantee: Jaqueline Cruz Geraldis
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Gastric cancer is the third leading cause of cancer death worldwide. Hypermethylation of tumor suppressor genes promoters represses transcription of these genes, conferring growth advantages to cancer cells. Our research group previously identified 83 differentially expressed genes in 5-aza-2'-deoxycytidine-treated gastric cancer cell lines compared with non-treated cells by microarray analysis. Among the upregulated genes identified in this analysis, MALAT1 and DRD5 were previously selected and evaluated for gene expression in paired neoplastic and adjacent non-neoplastic gastric tissue samples. Our data demonstrated lower levels of MALAT1 and DRD5 expression of in neoplastic tissues compared with non-neoplastic tissues, suggesting they are possible tumor suppressor genes in gastric cancer regulated by DNA methylation. The aim of the present study is to evaluate if MALAT1 and DRD5 promoter regions methylation are involved in transcriptional repression of these genes in gastric neoplastic tissues. For DNA methylation analysis, we will use the next generation sequencing technique, followed by the bioinformatic methods, in which mappers will be compared against their efficiency in suppling data within the methylation percentage in each CpG site. Therefore, this project will reveal specific regions responsible for the transcription regulation of genes poorly described in the gastric carcinogens, in Brazilian population, in order to contribute in the identification of new therapeutic's targets, once changes in DNA methylation patterns are potentially reversible. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WISNIESKI, FERNANDA; GERALDIS, JAQUELINE CRUZ; SANTOS, LEONARDO CAIRES; LEAL, MARIANA FERREIRA; CALCAGNO, DANIELLE QUEIROZ; GIGEK, CAROLINA OLIVEIRA; CHEN, ELIZABETH SUCHI; ANAUATE, ANA CAROLINA; ARTIGIANI, RICARDO; DEMACHKI, SAMIA; et al. ifferential regulation of LRRC37A2 in gastric cancer by DNA methylatio. Epigenetics, v. 17, n. 1, FEB 2021. (07/02470-3, 09/07145-9, 16/19953-6, 17/06227-8, 10/11174-1)

WISNIESKI, FERNANDA; GERALDIS, JAQUELINE CRUZ; SANTOS, LEONARDO CAIRES; LEAL, MARIANA FERREIRA; CALCAGNO, DANIELLE QUEIROZ; GIGEK, CAROLINA OLIVEIRA; CHEN, ELIZABETH SUCHI; ANAUATE, ANA CAROLINA; ARTIGIANI, RICARDO; DEMACHKI, SAMIA; et al. Differential regulation of LRRC37A2 in gastric cancer by DNA methylation. Epigenetics, v. 17, n. 1, p. 7-pg., 2021-02-07. (07/02470-3, 17/06227-8, 09/07145-9, 10/11174-1, 16/19953-6)