Morphofunctional changes in hepatic melanomacrophages of Physalaemus cuvieri (Anura: Leptodactylidae) induced by the contaminant benzo[alpha]pyrene.

• Grant number: 17/07971-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2017
• End date: January 01, 2020
• Field of knowledge: Biological Sciences - Zoology - Morphology of Recent Groups
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Classius de Oliveira
• Grantee: Lara Zácari Fanali
• Host Institution: Instituto de Biociências, Letras e Ciências Exatas (IBILCE). Universidade Estadual Paulista (UNESP). Campus de São José do Rio Preto. São José do Rio Preto , SP, Brazil
• Associated scholarship(s): 18/07989-1 - Effects of benzo[a]pyrene on the cytoskeleton and melanin dispersion of hepatic melanomacrophages of fish and amphibians, BE.EP.DR

Abstract

Anurans are vulnerable to various pollutants. Benzo[alpha]pyrene (BaP) is a contaminant with hepatotoxic, immunotoxic and genotoxic properties. Metabolized by the enzyme P450 (CYP1A1) produces toxic subproducts that can lead to the death of organisms. However, defense cells aid in the detoxification process. Melanomacrophages (MMs) are phagocytic cells that produce melanin, and the melanin granules can aggregate / disperse through the cytoskeleton (microtubules and actin filaments). According to the hypothesis that BaP is genotoxic, affects melanin production, microtubules and phagocytosis, our objectives will be: to evaluate the dispersion of the melanin area of hepatic MMs; Quantify the melanin production of MMs; To evaluate phagocytic activity of MMs, BaP changes in the cytoskeleton; Evaluate genotoxic effects. According to the hypothesis that ±-naphthoflavone (Anf), a CYP inhibitor, reduces subproducts of BaP, we aim to analyze how Anf acts, performing the same analyzes performed with BaP. The times will be 48 hours and 7 days, the doses will be 2mg / kg BaP and 20mg / kg Anf. There will be experiments with BaP; Tripan blue + BaP; Anf + BaP; Tripan blue + Anf + BaP. For the histological analyzes the procedures for inclusion in historesin and light microscopy analysis of the area of melanin and phagocytosis (tripan blue) will be followed. The amount of melanin will be measured by ELISA. For microtubules, pellets with MMs will be analyzed under a fluorescence microscope. For nuclear abnormalities the blood will be extracted and the procedures for analysis will be followed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FRANCO-BELUSSI, LILIAN; FANALI, LARA ZACARI; DE OLIVEIRA, CLASSIUS. UV-B affects the immune system and promotes nuclear abnormalities in pigmented and non-pigmented bullfrog tadpoles. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 180, p. 109-117, MAR 2018. (17/07971-2, 14/00946-4, 15/12006-9)

FANALI, LARA ZACARI; STURVE, JOACHIM; DE OLIVEIRA, CLASSIUS. xposure of Physalaemus cuvieri (Anura) to benzo[a]pyrene and alpha-naphthoflavone: Morphofunctional effects on hepatic melanomacrophages and erythrocytes abnormalitie. Environmental Pollution, v. 285, SEP 15 2021. (18/01078-7, 17/07971-2, 13/02067-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FANALI, Lara Zácari. Morphofunctional changes in liver melanomacrophages of fish and amphibians induced by the contaminant benzo [a] pyrene. 2020. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências Letras e Ciências Exatas. São José do Rio Preto São José do Rio Preto.