Characterization of the metabolome of anti-phytopatogen microorganisms by Multidimensional Gas Chromatography and Simple / Tandem Mass Spectrometry (GC×GC-QMS and GC×GC-MS/MS)

• Grant number: 16/20547-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2017
• End date: January 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Fabio Augusto
• Grantee: Joao Raul Belinato de Souza
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis, AP.TEM
• Associated scholarship(s): 18/25729-7 - High throughput biocompatible SPME coatings for metabolomic analyses, BE.EP.DR

Abstract

This project aims to develop methodologies to elucidate metabolic profile of yeast isolates (Saccharomyces cerevisiae, etc.) and other microorganisms with proven potential as biocontrol agents of diseases caused by postharvest pathogens fungi (Penicillium digitatum and italicum, Endomyces geotrichum , etc.) in citrus fruit. The analytical tools to be used will be Comprehensive Two-dimensional Gas Chromatography(GC × GC) coupled with Mass Spectrometry and Tandem Mass Spectrometry (MS / MS) and ultrahigh efficient liquid chromatography (UPLC-MS). The initial target will be volatile and nonvolatile metabolomes yeast isolates previously selected as interesting by researchers at the Citrus Center of the Campinas Agronomic Institute (IAC-CC), which will be compared with similar isolated without antimycotic activity. The association of antipatógena activity or lack of gas chromatographic fingerprints will be applying exploratory chemometric tools of higher order (PARAFAC, M-PCA and others). The methods studied here may, depending on the project's progress and the availability of samples, be applied to other isolated and / or bodies with different characteristics. It is expected that the results can be used as a tool for comprehension of the biochemical processes associated with anti-phytopathogen action of the individual, as well as the identification of active compounds and strains associated with this activity for possible application as control agents of post-harvest diseases.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BELINATO DE SOUZA, JOAO R.; DIAS, FERNANDA F. G.; CALIMAN, JAQUELINE D.; AUGUSTO, FABIO; HANTAO, LEANDRO W.. Opportunities for green microextractions in comprehensive two-dimensional gas chromatography / mass spectrometry-based metabolomics - A review. Analytica Chimica Acta, v. 1040, p. 1-18, DEC 21 2018. (11/51896-9, 17/25490-1, 15/05059-9, 16/20547-2)

BELINATO, JOAO RAUL; COSTA, CARINA PEDROSA; ALMEIDA, ADELAIDE; ROCHA, SILVIA M.; AUGUSTO, FABIO. Mapping Aspergillus niger Metabolite Biomarkers for In Situ and Early Evaluation of Table Grapes Contamination. FOODS, v. 10, n. 11, NOV 2021. (16/20547-2)

BELINATO, JOAO RAUL; ACQUARO, JR., VINICIUS RICARDO; DIAS CAPPELINI, LUCIANA TERESA; AUGUSTO, FABIO. New prospects and problems in sample preparation methods for microbiome analysis. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 143, OCT 2021. (18/25729-7, 17/08038-8, 18/03956-1, 16/20547-2, 14/07037-0)

BELINATO DE SOUZA, JOAO RAUL; KUPPER, KATIA CRISTINA; AUGUSTO, FABIO. In vivo investigation of the volatile metabolome of antiphytopathogenic yeast strains active against Penicillium digitatum using comprehensive two-dimensional gas chromatography and multivariate data analysis. Microchemical Journal, v. 141, p. 204-209, SEP 2018. (16/20547-2)

BELINATO, JOAO RAUL; GRANDY, JONATHAN J.; KHALED, ABIR; SUAREZ, PAOLA ALEJANDRA ORTIZ; PAWLISZYN, JANUSZ. Overcoming matrix effects in the analysis of pyrethroids in honey by a fully automated direct immersion solid-phase microextraction method using a matrix-compatible fiber. Food Chemistry, v. 340, MAR 15 2021. (18/25729-7, 16/20547-2)

SILVA, EVANDRO; BELINATO, JOAO RAUL; PORTO, CARLA; NUNES, ESTELA; GUIMARAES, FRANCISMAR; MEYER, MAURICIO CONRADO; PILAU, EDUARDO JORGE. Soybean Metabolomics Based in Mass Spectrometry: Decoding the Plant's Signaling and Defense Responses under Biotic Stress. Journal of Agricultural and Food Chemistry, v. 69, n. 26, p. 7257-7267, JUL 7 2021. (16/20547-2)

MORAES BAZIOLI, JAQUELINE; BELINATO, JOAO RAUL; COSTA, JONAS HENRIQUE; AKIYAMA, DANIEL YURI; DE MORAES PONTES, JOAO GUILHERME; KUPPER, KATIA CRISTINA; AUGUSTO, FABIO; DE CARVALHO, JOAO ERNESTO; FILL, TAICIA PACHECO. Biological Control of Citrus Postharvest Phytopathogens. TOXINS, v. 11, n. 8, AUG 2019. (18/03670-0, 16/20547-2, 17/24462-4, 18/13027-8)

JOÃO RAUL BELINATO; JAQUELINE MORAES BAZIOLI; ALESSANDRA SUSSULINI; FABIO AUGUSTO; TAÍCIA PACHECO FILL. METABOLÔMICA MICROBIANA: INOVAÇÕES E APLICAÇÕES. Química Nova, v. 42, n. 5, p. 546-559, 2019-07-18. (16/20547-2, 18/03670-0, 17/24462-4)

BELINATO DE SOUZA, JOAO RAUL; KUPPER, KATIA CRISTINA; AUGUSTO, FABIO. In vivo investigation of the volatile metabolome of antiphytopathogenic yeast strains active against Penicillium digitatum using comprehensive two-dimensional gas chromatography and multivariate data analysis. Microchemical Journal, v. 141, p. 6-pg., 2018-09-01. (16/20547-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUZA, Joao Raul Belinato de. New chromatographic strategies for metabolomics and exogenous compounds analysis in microorganisms and food matrices. 2020. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.