SlGSNOR manipulation consequences on reactive oxygen and nitrogen species metabolism and on nitrated and nitrosylated proteins profiles along tomato fruit ripening

• Grant number: 17/17935-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2018
• End date: July 31, 2021
• Field of knowledge: Biological Sciences - Botany
• Principal Investigator: Maria Magdalena Rossi
• Grantee: Marta Rodríguez Ruiz
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 16/01128-9 - Light and hormonal regulation of nutritional quality in Solanum lycopersicum, AP.TEM

Abstract

S-Nitrosoglutathione (GSNO), an S-nitrosated derivative of glutathione, plays a central role in nitric oxide (NO) signaling and represents the main NO reservoir in living cells. Endogenous GSNO levels are controlled by the enzyme S-nitrosoglutathione reductase (GSNOR), which catalyzes the reduction of GSNO to oxidized glutathione and ammonium and consequently influences protein S-nitrosylation and cell redox homeostasis. Accumulating evidence indicates that components of the nitroxidative and oxidative metabolisms, cell redox status, as well as S-nitrosylation and nitration events, are involved in the signaling cascades controlling the development and ripening of fleshy fruits. In this context, this project aims to evaluate the impacts of modulating GSNO levels via fruit-specific SlGSNOR silencing or overexpression on the nitroxidative and oxidative metabolisms and the global transcriptional profile of tomato (Solanum lycopersicum) fruits.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZUCCARELLI, RAFAEL; RODRIGUEZ-RUIZ, MARTA; SILVA, FERNANDA O.; GOMES, LETICIA D. L.; LOPES-OLIVEIRA, PATRICIA J.; ZSOGON, AGUSTIN; ANDRADE, SONIA C. S.; DEMARCO, DIEGO; CORPAS, FRANCISCO J.; PERES, LAZARO E. P.; et al. Loss of S-nitrosoglutathione reductase disturbs phytohormone homeostasis and regulates shoot side branching and fruit growth in tomato. Journal of Experimental Botany, v. N/A, p. 20-pg., 2023-05-09. (18/16389-8, 20/03720-8, 21/05714-8, 16/01128-9, 18/06436-9, 16/02033-1, 17/17935-3)

ZUCCARELLI, RAFAEL; RODRIGUEZ-RUIZ, MARTA; LOPES-OLIVEIRA, PATRICIA J.; PASCOAL, GRAZIELI B.; ANDRADE, SONIA C. S.; FURLAN, CLAUDIA M.; PURGATTO, EDUARDO; PALMA, JOSE M.; CORPAS, FRANCISCO J.; ROSSI, MAGDALENA; et al. Multifaceted roles of nitric oxide in tomato fruit ripening: NO-induced metabolic rewiring and consequences for fruit quality traits. Journal of Experimental Botany, v. 72, n. 3, SI, p. 941-958, FEB 11 2021. (16/04924-0, 18/16389-8, 16/01128-9, 17/17935-3)