FUNCTIONAL ANALYSIS OF HPV-RELATED PROTEINS IN ORAL SQUAMOUS CELL CARCINOMA

• Grant number: 17/16347-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: February 08, 2018
• End date: January 31, 2019
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Luiz Paulo Kowalski
• Grantee: Marisol Miranda Galvis
• Supervisor: Cristiane Helena Squarize
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: University of Michigan, United States
• Associated to the scholarship: 16/03248-1 - Mass spectometry based proteomic in oral squamous cell carcinoma in young patients, BP.DR

Abstract

Although the prevalence of oral squamous cell carcinoma (OSCC) has increased worldwide in young patients, there is no consensus regarding its etiological factors. Nevertheless, previous studies have proposed that high-risk human papillomavirus (HR-HPV) may be a promoting agent of oral carcinogenesis in this age group. Preliminary results: Laser-capture microdissection followed by liquid chromatography coupled mass spectrometry were carry out in twenty HR-HPV positive and HR-HPV negative OSCC samples from young patients. 1,030 proteins were identified and 26 were differentially expressed and enriched for biological processes. Five from the 26 proteins were selected for further investigation. Aim: To study the biological function of proteins involved in mTOR pathway to elucidare their role in oral carcinogenesis of HPV-related OSCC. Methods: The human OSCC cell lines UPCI-SCC-154 (HVP-positive) and SCC-9 (HVP-negative) will cultured to evaluate the biological impact of these proteins in preclinical model considering: proliferation, adhesion, migration and invasion produced by the overexpression or inactivation, through of Western blot and quantitative real time-polymerase chain reaction analysis; invasion, migration and adhesion assays.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIRANDA-GALVIS, MARISOL; SOARES, CAROLINA CARNEIRO; CARNIELLI, CAROLINA MORETTO; BUTTURA, JAQUELINE RAMALHO; DE SA, RAISA SALES; KAMINAGAKURA, ESTELA; MARCHI, FABIO ALBUQUERQUE; LEME, ADRIANA FRANCO PAES; PINTO, CLOVIS A. LOPES; SANTOS-SILVA, ALAN ROGER; et al. New Insights into the Impact of Human Papillomavirus on Oral Cancer in Young Patients: Proteomic Approach Reveals a Novel Role for S100A8. CELLS, v. 12, n. 9, p. 20-pg., 2023-05-05. (16/03248-1, 17/16347-0)

GALVIS, MARISOL MIRANDA; BORGES, GABRIEL ALVARES; DE OLIVEIRA, THIAGO BUENO; DE TOLEDO, ISABELA PORTO; CASTILHO, ROGERIO MORAES; SILVA GUERRA, ELIETE NEVES; KOWALSKI, LUIZ PAULO; SQUARIZE, CRISTIANE HELENA. Immunotherapy improves efficacy and safety of patients with HPV positive and negative head and neck cancer: A systematic review and meta-analysis. CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, v. 150, JUN 2020. (16/03248-1, 17/16347-0)