Proteome of saliva and acquired pellicle and its correlation with the microbiome of supra and subgingival biofilms in aggressive periodontitis

• Grant number: 17/26073-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: March 12, 2018
• End date: February 28, 2019
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Marcia Pinto Alves Mayer
• Grantee: Pâmela Pontes Penas Amado
• Supervisor: Walter Luiz Siqueira
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Institution abroad: Western University , Canada
• Associated to the scholarship: 15/00259-0 - Microbial diversity of supra and subgingival biofilms and protein profile of saliva and acquired pellicle of subjects with aggressive periodontitis, BP.DR

Abstract

Initial adhesion of bacteria to the dental surface is dependent on the acquired enamel pellicle, formed by proteins present in whole saliva. Studies on the microbiology of aggressive periodontitis indicate an increased proportion of periodontopathogens such as A.actinomycetemcomitans and P. gingivalis, but also decreased proportions of early colonizers such as streptococci and other beneficial organisms such as Actinomyces. The present study aims to test the hypothesis that the oral microbiota in aggressive periodontitis is modulated by differences in the acquired pellicle and saliva. The proteome of whole saliva (stimulated and unstimulated), parotid saliva and acquired pellicle will be evaluated by mass spectrometry. Samples were obtained from of 7 patients with localized aggressive periodontitis and 7 control subjects with healthy periodontium, paired by age, gender and race. Samples will be prepared and in-solution digestion will be performed. Peptide separation and mass spectrometric analyses will be carried out by LC-ESI-MS/MS (Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry). The obtained MS/MS spectra will be searched against human protein databases (Swiss Prot and TrEMBL, Swiss Institute of Bioinformatics, Geneva, Switzerland, http://ca.expasy.org/sprot/). (AU)