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Comparative study of monotherapy versus combination therapy with alendronate and/or Equisetum giganteum in osteoporotic rats submitted to femoral osteotomy: evidence for RANK/RANKL/OPG mechanism

Grant number: 18/00152-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2018
Effective date (End): December 31, 2018
Field of knowledge:Biological Sciences - Morphology - Histology
Principal researcher:Jose Angelo Camilli
Grantee:Bruna Bighetto Cain
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The present project represents a deepening of the scientific study with the hydroalcoholic extract of Equisetum giganteum (EHEg), carried out in the project "PIBIC - QUOTA 2016/2017", due to the excellent results and the unprecedented aspects associated to them, being even awarded in the 4th Bioscience and Technology Conference (BITEC 2017) as "Best Panel Presentation". Natural products have been recognized as effective drugs for a wide variety of therapeutic indications. The medicinal plant Equisetum giganteum has been empirically used for a long time in Brazil for bone repair and/or osteoporosis treatment, however, the literature lacks scientific studies to prove these activities. Sodium alendronate (ALE), a bisphosphonate and therefore an antireabsorbing drug, is recognized as one of the "gold standard" reference drugs for the treatment of osteoporosis in Brazil and is widely distributed in the Brazilian Unified Health System (SUS), however, it causes a number of significant side-effects. In this context, this new stage of the study aims to evaluate the therapeutic potential of monotherapy (ALE or EHEg) and combined therapy (ALE+EHEg) in osteoporotic female rats using low doses (1 mg/kg, i.g.) of ALE and/or EHEg, preventing chronic treatment from inducing significant side-effects. Through a pre-clinical experimental design, the present project will investigate behavioral, morphological and toxicological aspects, as well as the mechanism of bone remodeling through RANK/RANKL/OPG system investigation. Thus, this study may support the inclusion of this plant species in the list of medicinal plants of Resolution-RDC10/2010, ordinance 971 of the PNPIC of the Brazilian Ministry of Health, as well as highlight a possible synergic or summation effect as a result of the combined treatment (ALE+EHEg) for the treatment of osteoporosis and/or for bone repair without inducing significant side-effects. (AU)