Evaluation of bone healing at the bone-implant interface in rats with induced osteoporosis treated with raloxifene or alendronate - Histometric, immunohistochemical, biomechanical, and microscopy epifluorescence analysis.

• Grant number: 12/15748-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): November 01, 2012
• Effective date (End): February 28, 2013
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Roberta Okamoto
• Grantee: Gabriel Ramalho Ferreira
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This project aims to evaluate the bone repair process at the bone / implant interface in rats with induced osteoporosis and treated with raloxifene or alendronate submitted to histometric, immunohistochemical, epifluorescence microscopy and biomechanical analysis (reverse torque). For this, 80 rats (Rattus norvegicus Albinus, Wistar), weighing 200 grams will be divided into three groups according to the analysis to be submitted, subdivided into four other subgroups according to -induced osteoporosis and drug treatment (SHAM BD = Rats that underwent sham surgery and were fed with a balanced diet; OVX WT = rats that underwent bilateral ovariectomy and fed with a low calcium diet (osteoporotic) without drug treatment; OVX RAL = rats submitted to bilateral ovariectomy and fed with a low calcium diet ( osteoporotic), treated with raloxifene; OVX ALE = rats submitted to bilateral ovariectomy and fed with a low calcium diet (osteoporotic), treated with alendronate sodium). Each animal will receive two implants, one in each tibial metaphysis. The implants that will be installed in the right tibia with will have a machined surface, and the left tibia will receive an implant with modified surface by double acid-etching. Euthanasia of the animals shall be carried out in the periods of 14, 42 and 60 days after implants installation, depending on the analysis to be made by means of an overdose of anesthesia. Euthanasia periods are determined in accordance with the analysis to be performed. For the histometric (stained with hematoxylin and eosin) and immunohistochemical analysis, 20 rats will be euthanized at 14 days and 20 rats at 42 days after implant placement. For epifluorescence microscopy analysis, 20 rats will be euthanized at 60 days postoperatively. And for the biomechanical analysis (reverse torque), 20 rats will be euthanized at 42 days postoperatively. All data will be submitted to a normality curve for determining the most appropriate test (parametric versus nonparametric). The significance level of 0.05 will be adopted for all tests.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FAVERANI, LEONARDO PEREZ; BRAGA POLO, TARIK OCON; RAMALHO-FERREIRA, GABRIEL; CORREA MOMESSO, GUSTAVO ANTONIO; HASSUMI, JAQUELINE SUEMI; ROSSI, ANA CLAUDIA; FREIRE, ALEXANDRE RODRIGUES; PRADO, FELIPPE BEVILACQUA; LUVIZUTO, ELOA RODRIGUES; GRUBER, REINHARD; et al. Raloxifene but not alendronate can compensate the impaired osseointegration in osteoporotic rats. CLINICAL ORAL INVESTIGATIONS, v. 22, n. 1, p. 255-265, JAN 2018. (12/15912-2, 12/15748-8)

RAMALHO-FERREIRA, G.; FAVERANI, L. P.; PRADO, F. B.; GARCIA, JR., I. R.; OKAMOTO, R.. Raloxifene enhances peri-implant bone healing in osteoporotic rats. International Journal of Oral and Maxillofacial Surgery, v. 44, n. 6, p. 798-805, JUN 2015. (12/15912-2, 12/15748-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FERREIRA, Gabriel Ramalho. Avaliação do reparo ósseo na interface osso/implante em ratas com osteoporose induzida tratadas com raloxifeno ou alendronato: análise histométrica, imunoistoquímica, por epifluorescência e biomecânica. 2014. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.