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Investigation of cell surface proteome of murine melanocytes treated with tumor secretome

Grant number: 17/22330-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): March 01, 2018
Effective date (End): December 31, 2018
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:André Zelanis Palitot Pereira
Grantee:Rafael Berton Correia Ramos
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The search for biomarkers has been the focus of a number of studies in tumor biology, however, given the etiological diversity of factors associated with oncogenesis, few candidates have been identified so far. The imbalance in cellular homeostasis caused by oncogenesis has an expressive effect on the repertoire of proteins present in tumor cells, as well as on the physiological events resulting from the interaction of proteins with the cell surface surface. In the last decade, large-scale analysis and acquisition of data (so-called 'omics' approaches) have been used in order to understand the diversity of proteins secreted by cells (cell secretomes) within different biological contexts. Thus, the main goal of this project is to use high resolution mass spectrometry and proteomic analysis to investigate the effect of stimulation of normal murine melanocytes with tumoral secretome by evaluating the repertoire of cell surface proteins before and after the stimulus, as well as integrating the results with biological pathways relevant to the oncogenic process. (AU)