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Proteolytic processing analysis in the secretome of melanoma tumor cells

Grant number: 17/07897-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): June 01, 2017
Effective date (End): July 31, 2018
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:André Zelanis Palitot Pereira
Grantee:Isabella Fukushima
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil
Associated research grant:14/06579-3 - System-wide analysis of N-terminal processing and protein diversity in the secretome of tumor cells, AP.JP
Associated scholarship(s):17/24560-6 - Proteolytic processing analysis in the secretome of melanoma tumor cells, BE.EP.IC


The search for biomarkers has been addressed by several investigators in the field of cancer biology, however, given the multitude of etiologic factors associated to oncogenesis, a number of challenges still need to be overcome. The imbalance of cellular homeostasis during oncogenesis has a marked effect on the repertoire of the proteins secreted by malignant cells (cellular secretome), as well as, upon the physiological processing events that occurs during or after protein synthesis. The development and improvement of high throughput analytical techniques provide main evidences regarding the complexity in ordinary physiological mechanisms of eukaryotic cells, as well as pathological processes such as cancer. In this context, proteolytic processing is responsible for the generation of new protein N-termini and comprises an essentially irreversible cell signaling process, affecting a number of biological pathways with important implications in oncogenesis. Therefore, the main goal of this project is to identify the peptidome diversity derived from proteolytic processing events in the secretome of cultured tumor cells correlating the results with biological pathways that might be eventually involved in the oncogenesis process. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LIBERATO, TARCISIO; FUKUSHIMA, ISABELLA; KITANO, EDUARDO S.; SERRANO, SOLANGE M. T.; CHAMMAS, ROGER; ZELANIS, ANDRE. Proteomic profiling of the proteolytic events in the secretome of the transformed phenotype of melanocyte-derived cells using Terminal Amine Isotopic Labeling of Substrates. JOURNAL OF PROTEOMICS, v. 192, p. 291-298, . (14/06579-3, 13/07467-1, 17/07897-7)
LIBERATO, TARCISIO; PESSOTTI, DAYELLE S.; FUKUSHIMA, ISABELLA; KITANO, EDUARDO S.; SERRANO, SOLANGE M. T.; ZELANIS, ANDRE. Signatures of protein expression revealed by secretome analyses of cancer associated fibroblasts and melanoma cell lines. JOURNAL OF PROTEOMICS, v. 174, p. 1-8, . (13/07467-1, 14/06579-3, 17/07897-7)
ZELANIS, ANDRE; SILVA, DEBORA A.; KITANO, EDUARDO S.; LIBERATO, TARCISIO; FUKUSHIMA, ISABELLA; SERRANO, SOLANGE M. T.; TASHIMA, ALEXANDRE K.. A first step towards building spectral libraries as complementary tools for snake venom proteome/peptidome studies. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 31, . (13/14651-3, 14/06579-3, 17/20106-9, 13/07467-1, 17/07897-7, 13/13548-4)

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