Prospection of markers associated to proteolytic processing in plasma samples of patients with melanoma

• Grant number: 19/07282-8
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2020
• End date: August 31, 2022
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: André Zelanis Palitot Pereira
• Grantee: André Zelanis Palitot Pereira
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil
• Associated researchers: Adriana Franco Paes Leme ; Miyuki Uno ; Roger Chammas

Abstract

Melanoma is an aggressive skin cancer and a lethal melanocytic neoplasm, with increasing annual number of cases (faster than any other solid tumor). Since the protein composition (proteome) of tumoral cells may be regarded as a description of cellular status, the evaluation of protein expression patterns in biopsy samples may lead to the discovery of cancer-related markers and new drug targets. In the context of tumoral development, proteolytic processing plays an important role, favoring the dispersion of tumor cells to distant sites (metastasis) as well as in mediating irreversible proteolytic signaling events. The main goal of this project is to probe the Biobank of the São Paulo of Cancer Institute (ICESP) aiming at validating prognostic markers that had already been identified in our previous research. Data obtained at this work will provide a systems-wide profile of proteolytic signaling in melanoma, with important translational potential, contributing to the understanding of the progression of melanoma. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GALLO, GLORIA; BARCICK, UILLA; COELHO, CAMILA; SALARDANI, MURILO; CAMACHO, MAURICIO F.; CAJADO-CARVALHO, DANIELA; LOURES, FLAVIO V.; SERRANO, SOLANGE M. T.; HARDY, LEON; ZELANIS, ANDRE; et al. A proteomics-MM/PBSA dual approach for the analysis of SARS-CoV-2 main protease substrate peptide specificity. Peptides, v. 154, p. 8-pg., 2022-08-01. (13/07467-1, 19/07282-8)

ZELANIS, ANDRE; BARCICK, UILLA; RACORTI, NATHALIA DE VASCONCELLOS; SALARDANI, MURILO. Heterotypic communication as the promoter of phenotypic plasticity of cancer cells: The role of cancer secretomes. PROTEOMICS, v. N/A, p. 10-pg., 2023-07-20. (21/05087-3, 19/07282-8)

DE MORAIS, JULIANA A.; ZELANIS, ANDRE. Bioinformatic reanalysis of public proteomics data reveals that nuclear proteins are recurrent in cancer secretomes. TRAFFIC, v. 23, n. 2, DEC 2021. (19/07282-8, 14/06579-3)

SALARDANI, MURILO; BARCICK, UILLA; ZELANIS, ANDRE. Assessing proteolytic events in bioinformatic reanalysis of public secretome data from melanoma cell lines. BIOCHEMISTRY AND BIOPHYSICS REPORTS, v. 30, p. 4-pg., 2022-07-01. (14/06579-3, 19/07282-8)

CAMACHO, MAURICIO FROTA; STUGINSKI, DANIEL R.; ANDRADE-SILVA, DEBORA; NISHIYAMA-JR, MILTON Y.; VALENTE, RICHARD H.; ZELANIS, ANDRE. A snapshot of Bothrops jararaca snake venom gland subcellular proteome. Biochimie, v. 214, p. 10-pg., 2023-06-17. (14/06579-3, 19/07282-8)

PESSOTTI, DAYELLE S.; ANDRADE-SILVA, DEBORA; SERRANO, SOLANGE M. T.; ZELANIS, ANDRE. Heterotypic signaling between dermal fibroblasts and melanoma cells induces phenotypic plasticity and proteome rearrangement in malignant cells. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1868, n. 12, p. 9-pg., 2020-12-01. (14/06579-3, 19/07282-8, 16/16935-7, 13/07467-1)