PiRNA actuation in transposable elements silencing on B chromosome presence of the Astatotilapia latifasciata (Cichlidae)

• Grant number: 17/25193-7
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2018
• End date: February 28, 2022
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: César Martins
• Grantee: Jordana Inácio Nascimento Oliveira
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 15/16661-1 - Sex and B chromosome enigmas: model systems for the study of chromosome and genome evolution, AP.TEM

Abstract

The supernumerary chromosomes B (Bs) are found in several eukaryotic organisms, does not follow the mendelian heritage and has its own transmission mechanism (drive), which is less understood under molecular point view. The Bs genomic constitution shows a mosaics sequences, which includes repetitives DNA, genes and pseudogenes. Preview studies had related this element to several functions, as genetic variability among populations, fertility effects, adaptation and evolutive link to sexual chromosomes origin. It is know which in Astatotilapia latifasciata African cichlid fish the B chromosome is enriched of repetitive DNA sequences, mainly Transposable Elements (TEs), being that some this are differentially expressed. Additionally, to this cichlid transcriptome and microRNome large scale data had revealed differential transcriptional activity among sexes and, among B minus and B plus individuals. Further, a copy of pdl6 with high integrity was identified in B chromosome. This gene is involved piwiRNAs biogenesis (piRNAs), which promote transposable elements silencing and ensure the integrity of genome in gonads tissue. Therefore, either one TEs or the other B chromosome are known as selfish elements it is suggested which piRNAs activity be differentially regulated in B presence. Thus, the perspectives to investigation under functional focus shows quite promising to the understanding of B chromosome behavior and influences in environmental cell. This work proposes to investigate the piRNAs and TEs activity in gonads tissue of the A. latifasciata in B chromosome presence. Therefore, using the transcriptome, small RNAs sequencing and genome of A. latifasciata existing data will be performed in silico prediction analysis, piRNAs and protein expression assay related to piRNAs biogenesis and transposition. Lately, the in silico analysis obtained results are validated by expression and physics localization analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, JORDANA INACIO NASCIMENTO; CARDOSO, ADAUTO LIMA; WOLF, IVAN RODRIGO; DE OLIVEIRA, ROGERIO ANTONIO; MARTINS, CESAR. First characterization of PIWI-interacting RNA clusters in a cichlid fish with a B chromosome. BMC Biology, v. 20, n. 1, p. 18-pg., 2022-09-21. (15/16661-1, 16/09320-6, 17/25193-7)

NASCIMENTO-OLIVEIRA, JORDANA INACIO; FANTINATTI, BRUNO EVARISTO ALMEIDA; WOLF, IVAN RODRIGO; CARDOSO, ADAUTO LIMA; RAMOS, ERICA; RIEDER, NATHALIE; DE OLIVEIRA, ROGERIO; MARTINS, CESAR. Differential expression of miRNAs in the presence of B chromosome in the cichlid fish Astatotilapia latifasciata. BMC Genomics, v. 22, n. 1, MAY 12 2021. (15/16661-1, 17/25193-7, 16/09320-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Jordana Inácio Nascimento. Small non-coding RNAs (microRNAs and PIWI-interacting RNAs) characterization in the B chromosome presence in Astatotilapia latifasciata. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.