Laparoscopic cholecystectomy using vessel sealant device and modification of technique using hand tied loop in rabbits: experimental study

• Grant number: 17/23402-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2018
• End date: July 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Paola Castro Moraes
• Grantee: Monica Carolina Nery Wittmaack
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Laparoscopic surgeries have known advantages being widely used in human medicine and there is a great tendency to reduce open surgical procedures. However, in veterinary medicine, this advancement in video-surgeries does not occur in the same way. Minimally invasive surgeries are still restricted to some centers, with little research that seeks to advance techniques in small animals. Laparoscopic cholecystectomy is a recent minimally invasive surgical technique in veterinary medicine. Randomized, live studies evaluating different methods of laparoscopic extrahepatic bile duct ligation and dissection bring benefits to patients. The purpose of our study was to describe two techniques of laparoscopic cholecystectomy and to compare two methods of occlusion of the cystic duct, reporting complications and trans and postoperative results, as well as proposing a modification of the technique of laparoscopic cholecystectomy. Thus, 20 rabbits will be used as an experimental model. Laparoscopic cholecystectomy technique by gallbladder dissection prior to cystic duct dissection and hand tied loop application (n = 10), and laparoscopic cholecystectomy technique using vessel sealant device for cystic duct ligation through four portals (n = 10). The total time of surgery and each operative stage will be measured for the proposed techniques. Serial ultrasonographic evaluations will be performed postoperatively in order to verify the absence of biliary leakage and biliary peritonitis. Abnormal macroscopic changes will be verified at the fifteenth postoperative day by means of the necropsy of the twenty animals, and the vessel-sealing device enables adequate occlusion of the cystic duct comparable to the hand tied loop. We hypothesized that there would be no difference between the quality of the seal between the vessel sealant device and the hand tied loop for cystic duct ligation and we expect that the modification of the laparoscopic cholecystectomy technique by performing the gallbladder dissection prior to the cystic duct will be a viable and safe option. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MONICA CAROLINA NERY WITTMAACK; MARIA EDUARDA BASTOS ANDRADE MOUTINHO CONCEIÇÃO; MARÍA CAMILA MALDONADO VERA; RACHEL INAMASSU FACCINI; GUILHERME SEMBENELLI; GABRIEL LUIZ MONTANHIM; MARELIZA POSSA DE MENEZES; FABIANA DEL LAMA ROCHA; LUIZ PAULO NOGUEIRA AIRES; PAOLA CASTRO MORAES. Comparative evaluation of three laparoscopic cholecystectomy techniques in rabbit’s model. Acta Cirurgica Brasileira, v. 38, 2023-12-01. (17/23402-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

WITTMAACK, Monica Carolina Nery. Comparative evaluation of three laparoscopic cholecystectomy techniques in rabbits’ model. 2021. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.