Effects of flavonoid sakuranetin administration in pulmonary alterations induced by pollutant instillation (total suspended particles) in mice

• Grant number: 17/23729-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2018
• End date: December 31, 2019
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Carla Máximo Prado
• Grantee: Stheffany de Oliveira Santos
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

The emission of pollution into the atmosphere increases the amount of airborne particles. These particles can cause harmful effects to human health due to their small diameter, which facilitates their passage into the distal airways, causing pulmonary inflammation and also systemic inflammation. The use of molecules, obtained through medicinal plants, has been widely studied with the potential to develop new therapeutic strategies in several diseases, including respiratory ones. In this sense, our group has studied sakuranetin, a flavonoid extracted in large quantities in plants of the genus Baccharis. Previous studies have identified anti-inflammatory and antioxidant properties in this substance. Aim: To evaluate the effects of administration of sakuranetin on pulmonary inflammation in mice submitted to instillations of particles of pollution. For this purpose, animals will receive instillation of these particles at a concentration of 30 ¼g diluted in 10 ¼l for 7 or 14 days and receive sakuranetin for the same period at a dose of 20 mg / kg. After this period the animals will be anesthetized and submitted to lung function evaluation, euthanized and collected bronchoalveolar lavage for inflammatory cell counting and lung and nose will be removed for morphometric analysis by histological techniques. The statistical analysis will be performed through the Sigma Plot Program, and the test used will be later decided based on the data distribution. Expected results: In view of the anti-inflammatory potential of sakuranetin already observed in other studies of our group, our hypothesis is that the administration of continuous sakuranetin reduces the effects of instillation of pollutants in the lungs of these animals. (AU)