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Effect of Violet LED (405-410nm) application on dentin structure

Grant number: 18/03983-9
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): May 07, 2018
Effective date (End): May 06, 2019
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal researcher:Patricia Moreira de Freitas Costa e Silva
Grantee:Eric Mayer dos Santos
Supervisor abroad: Lorenzo Breschi
Home Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: Università di Bologna, Italy  
Associated to the scholarship:16/24126-1 - long-therm clinical evaluation of in-office dental bleaching using a violet light (led 405-410 nm), BP.DR


A recent technique using a violet LED (405-410 nm) has been introduced as an alternative for the conventional bleaching treatment. This study aims to evaluate and compare the effects of different bleaching techniques on the dentin structure. Twenty blocks of human dentin (n=4) will be randomly divided in 5 groups, as follows: G1: no treatment (control group); G2: application of 35% hydrogen peroxide for 45 minutes; G3: irradiation with violet LED (405-410 nm) for 20 minutes; G4: violet LED irradiation for 20 minutes followed by the application of 35% hydrogen peroxide for 20 minutes and then the violet LED irradiation for 20 minutes (hybrid technique); G5: application of hydrogen peroxide 35% + violet LED irradiation for 20 minutes (at the same time). Zymography and in situ zymography techniques will be performed to determine Matrix Metalloproteinase and dentin protein content. Statistical analyses will be performed considering a significant level of 5%.

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