Could physical exercise improve visual acuity? Effects of different physical training protocols on tear composition and clusterin/ApoJ metabolism in the retina of obese rodent

• Grant number: 18/06833-8
• Support Opportunities: Scholarships in Brazil - Master
• Start date: May 01, 2018
• End date: April 30, 2020
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Leandro Pereira de Moura
• Grantee: Thaís Dantis Pereira de Campos
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 15/07199-2 - Role of clusterin/ApoJ on insulin signalling in response to physical exercise in rodents and humans, AP.JP

Abstract

Obesity is a multifactorial disease, chronic and non-transmissible, defined by the accumulation of adipose tissue that is commonly associated with systemic inflammation. This state is linked to health damage and has increased its incidence worldwide. This disorder has been correlated as responsible for the appearance of various eye diseases and consequently damage to visual acuity. More specifically, cardiometabolic risk, due to the progression of the inflammatory profile, has been shown to be associated with lacrimal gland dysfunction, implicating in the development and aggravation of Cerato-Conjunctivitis Sicca or popularly known as dry eye disease. In more severe and extreme cases, the condition of obesity and inflammation can trigger the total loss of vision. In this scenario, in addition to its numerous metabolic functions, clusterin has been described as protective of the ocular surface by a mechanism of seal, which assists in cell survival. On the other hand, it is known that physical exercise can act as an important non-pharmacological tool in the fight against inflammation and obesity. However, no study was pereformed in order to investigate whether regular practice of different physical exercise protocols can modulate the tear composition and the clusterin signaling pathway in the retina and thus promote an improvement in visual acuity. Thus, the present study aims to elucidate the role of different chronic protocols of physical exercises (aerobic, strength and combined) in the levels of clusterin in the tear and to evaluate the metabolism of clusterin in the retina of obese mice. As preliminary results, through bioinformatics analyzes, we observed that there are the genes of clusterin and its receptor LRP1 / 2 in the eye and retina. Moreover, with the project already in progress, we observed that obese and insulin-resistant mice present increased gene expression of TNF-± and IL-1² and also elevated levels of these proinflammatory molecules in the retina. In addition, it was possible to observe that these animals presented reduction of clusterin levels also in the retina. After the conclusion of this study, it is expected to discover if the physical exercise is capable of promoting changes in the ocular system and if yes, unraveling through the metabolism of clusterin, this practice can improve visual acuity. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE CAMPOS, THAIS DANTIS PEREIRA; DA CRUZ RODRIGUES, KELLEN CRISTINA; PEREIRA, RODRIGO MARTINS; ANARUMA, CHADI PELLEGRINI; DOS SANTOS CANCIGLIERI, RAPHAEL; DE MELO, DIEGO GOMES; DA SILVA, ADELINO SANCHEZ RAMOS; CINTRA, DENNYS ESPER; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO; et al. The protective roles of clusterin in ocular diseases caused by obesity and diabetes mellitus type 2. MOLECULAR BIOLOGY REPORTS, v. 48, n. 5, p. 4637-4645, MAY 2021. (18/06833-8, 15/07199-2)

PEREIRA DE CAMPOS, THAIS DANTIS; DA CRUZ RODRIGUES, KELLEN CRISTINA; PEREIRA, RODRIGO MARTINS; MORELLI, ANA PAULA; DA ROCHA, ALISSON LUIZ; CANCIGLIERI, RAPHAEL DOS SANTOS; RAMOS DA SILVA, ADELINO SANCHEZ; ROPELLE, EDUARDO ROCHETE; PAULI, JOSE RODRIGO; SIMABUCO, FERNANDO MOREIRA; et al. Short-Term Combined Exercise Improves Inflammatory Profile in the Retina of Obese Mice. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 17, SEP 2020. (15/07199-2, 18/06833-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CAMPOS, Thaís Dantis Pereira de. The role of clusterin/ApoJ on the ocular surface in experimental models of obesity: implications of different physical training protocols. 2020. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Aplicadas Limeira, SP.