Generation of translocated chromosomes probes of the Mazama gouazoubira species by microdissection

• Grant number: 18/06780-1
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: July 01, 2018
• End date: August 31, 2018
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: José Maurício Barbanti Duarte
• Grantee: Agda Maria Bernegossi
• Supervisor: Vladimir Trifonov
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Institution abroad: Siberian Branch of the Russian Academy of Sciences (SB RAS), Russia
• Associated to the scholarship: 17/21333-9 - Preparation of chromosome translocations probes of Mazama gouazoubira species by microdissection, BP.IC

Abstract

The Brazilian species of neotropical deer Mazama gouazoubira (2n = 70; NF = 70), also known as gray brocket deer, is characterized by having retained the ancestral karyotype of the Cervidae family and for presenting frequent Robertsonian translocations. A recent study, using the technique of band pattern G, has identified in the Brazilian population of M. gouazoubira seven distinct types of translocations (X; 16 X; 21, 7; 21, 8; 21, 4; 16, 20; 26, 14; 16), involving nine different chromosomes. However, a confirmation of the sharing of a same type of translocation by different animals, through the technique of fluorescent in situ hybridization (FISH) was unable due to the difficulty on the techniques of molecular cytogenetics. As such, the present study seeks the use of advanced techniques on the detection and confirmation of the chromosomal rearrangements that have occurred in the gray brocket deer. In order to do so this study has the objective of preparing probes by micro-dissection of translocated chromosomes from individuals that have presented different types of translocations. To confirm if the same type of translocation is shared by different animals, the manufactured probes will be used in FISH technique. In addition, these probes will be hybridized in individuals without Robertsonian translocations, aiming for chromosome markers establishment to the M. gouazoubira species since its chromosomes presents similar morphology and size, making the individualization and differentiation of each chromosome pair difficult. It is expected that the results at the end of this study contributes as a baseline for subsequent studies, enabling to solve doubts related to karyotype evolution of the Cervidae family. (AU)