Protein gene expression by stem cells from pulp of deciduous teeth after the use of Low Intensity Laser

• Grant number: 17/11396-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2018
• End date: March 31, 2020
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Mariel Tavares de Oliveira Prado Bergamo
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated scholarship(s): 18/13675-0 - Dental pulp stem cell biology., BE.EP.DR

Abstract

This study aimed to evaluate the effect of different energy densities of Low Intensity Laser on cell viability and proliferation of stem cell from human exfoliated deciduous teeth and dental pulp cells; on the RNAm expression of FGF2, VEGF, DSPP and DMP-1; and on the protein expression of FGF2, VEGF, DSPP and DMP-1. Cells samples will be obtained and grown in MEM± e DMEM culture medium with Fetal Bovine Serum (FBS). After the fourth passage, the sample will be irradiated with low intensity laser to the following groups: Group 1(1.2 J / cm2 - 05 mW - 10s), Group 2(2.5 J / cm2 - 05 mW - 20s), Group 3(3.7 J / cm2 - 05 mW - 30s), Group 4(5.0 J / cm2 - 05 mW - 40s), Group 5(6.2 J / cm2 - 05 mW - 50s), Group 6(2.5 J / cm2 - 10 mW - 10s), Group 7(3.7 J / cm2 - 15 mW - 10s), Group 8( 5.0 J / cm2 - 20 mW - 10 sec), Group 9 (6.2 J / cm2 - 25 mW - 10 sec), Positive Control (MEM± +10% FBS - no irradiated), Negative Control (MEM± +1% FBS - no irradiated). The techniques used for the analysis will be MTT, Crystal Violet, RT-PCR, ELISA, Western Blot and Immunofluorescence. The values in triplicate will be analyzed by ANOVA followed by Tukey test and Kruskal-Wallis analysis complemented by Dunn's test when obey normal and non-normal distribution, respectively. The significance level of 5% will be adopted for the differences considered statistically significant.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

OLIVEIRA PRADO BERGAMO, MARIEL TAVARES; RIBEIRO VITOR, LUCIANA LOURENCO; LOPES, NATHALIA MARTINS; LOURENCO NETO, NATALINO; DIONISIO, THIAGO JOSE; OLIVEIRA, RODRIGO CARDOSO; RODINI, CAMILA OLIVEIRA; SANTOS, CARLOS FERREIRA; ANDRADE MOREIRA MACHADO, MARIA APARECIDA; OLIVEIRA, THAIS MARCHINI. Angiogenic protein synthesis after photobiomodulation therapy on SHED: a preliminary study. Lasers in Medical Science, v. 35, n. 9, FEB 2020. (17/11396-3, 18/20316-6)

BERGAMO, MARIEL TAVARES; VITOR, LUCIANA LOURENCO RIBEIRO; DIONISIO, THIAGO JOSE; MARQUES, NADIA CAROLINA TEIXEIRA; OLIVEIRA, RODRIGO CARDOSO; AMBROSIO, ELOA CRISTINA PASSUCCI; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; LOURENCO NETO, NATALINO; MACHADO, MARIA APARECIDA ANDRADE MOREIRA; et al. Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?. Lasers in Medical Science, APR 2021. (18/20316-6, 17/11396-3)

BERGAMO, MARIEL TAVARES; VITOR, LUCIANA LOURENCO RIBEIRO; DIONISIO, THIAGO JOSE; MARQUES, NADIA CAROLINA TEIXEIRA; OLIVEIRA, RODRIGO CARDOSO; AMBROSIO, ELOA CRISTINA PASSUCCI; SAKAI, VIVIEN THIEMY; SANTOS, CARLOS FERREIRA; LOURENCO NETO, NATALINO; MACHADO, MARIA APARECIDA ANDRADE MOREIRA; et al. Could the photobiomodulation therapy induce angiogenic growth factors expression from dental pulp cells?. Lasers in Medical Science, v. 36, n. 8, p. 1751-1758, OCT 2021. (17/11396-3, 18/20316-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BERGAMO, Mariel Tavares de Oliveira Prado. Relation between the function and expression of VEGFR1 and the vasculogenic differentiation of dental pulp stem cells. 2020. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB) Bauru.