Impact of pregnancy-associated plasma protein- a (PAPP-A) addition during in vitro oocyte maturation on the profile and lipid metabolism of bovine embryos

• Grant number: 18/06454-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2018
• End date: November 30, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Anthony César de Souza Castilho
• Grantee: Victor Augusto Vieira de Lima
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
• Associated research grant: 13/11480-3 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cattle: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHr) expression, AP.JP

Abstract

The insulin-like growth factor (IGF), together with its binding protein (IGFBP), participate in lipid metabolism and adiposity regulation. A good regulator for this set is Pregnancy-Associated Plasma Protein A (PAPP-A) capable of breaking the bonds between IGF and its binding proteins increasing the bioavailability of free IGF, which leads to a reduction in the amount of lipids due to the increase in the rate of ² oxidation. Many studies have already investigated the action of exogenous IGF-I on in vitro maturation (IVM), but little was known about the effects of PAPP-A addition during this step; and its impact on quality and future embryonic metabolism. Recent data obtained by our group report that the addition of 100 ng/mL of PAPP-A during IVM has positive effects to embryo quality. Thus, evaluate the addition or modulation of the IGF composition and its availability are important researches. In this context, the objective of this research project is to investigate the effects of adding 100 ng/mL PAPP-A during IVM of cumulus-oocyte complexes (COCs) from slaughterhouse ovaries about: 1) the lipid content of blastocysts, by means of the Sudan-Black B coloration; 2) the phospholipid profile of blastocysts, through mass spectrometry analysis (MALDI-MS); and 3) the pattern of genes expression related to the lipid profile in embryos.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DELLAQUA, THAISY TINO; FRANCHI, FERNANDA FAGALI; DOS SANTOS, PRISCILA HELENA; GIROTO, ALAN BRUNHOLI; NUNES, SARAH GOMES; DE LIMA, VICTOR AUGUSTO VIEIRA; GUILHERME, VITORIA BERTOGNA; FONTES, PATRICIA KUBO; SUDANO, MATEUS JOSE; CASTILHO, ANTHONY CESAR DE SOUZA. Molecular phenotypes of bovine blastocyst derived from in vitro-matured oocyte supplemented with PAPP-A. VETERINARY RESEARCH COMMUNICATIONS, v. N/A, p. 10-pg., 2023-01-18. (17/19679-4, 18/06454-7)