Efficacy assessment of photoprotective formulations containing UV filters and quercetin-loaded solid lipid nanoparticles in reconstructed skin model

• Grant number: 18/10481-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: July 14, 2018
• End date: March 31, 2019
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Lorena Rigo Gaspar Cordeiro
• Grantee: Camila Martins Kawakami
• Supervisor: Monika Schafer-Korting
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Freie Universität Berlin, Germany
• Associated to the scholarship: 15/10017-3 - Efficacy and safety assessment of photoprotective formulations in reconstructed human skin: effect of photostability on markers related to photoaging and skin cancer, BP.DR

Abstract

It is well known that the regular application of sunscreens is the most effective approach to prevent the harmful effects of UV radiation, such as sunburn, immunosuppression, photoaging, DNA damage and cancer. In addition, there has recently been a tendency to use natural compounds, such as polyphenols, in order to improve the potential of sunscreens to prevent UV-induced damages. Sunscreens formulated with antioxidants may have synergistic photoprotective effects when compared with these agents alone, covering all mechanisms involved beyond UV radiation. Nowadays, there are several studies in the literature demonstrating the efficacy of photoprotective formulations using cell culture, in vivo models and also human volunteers; however, there are few studies employing reconstructed skin model, which replace animal experimentation and are more relevant since they use human cells. Therefore, this study is relevant since it aims to assess efficacy of photoprotective formulations containing UV filters and quercetin-loaded solid lipid nanoparticles (SLN) using a reconstructed human skin model. For this purpose, two sunscreen formulations (containing or not quercetin-loaded SLN) will be applied in the reconstructed skin models and will be submitted to the detection of cyclobutane pyrimidine dymmers (CPDs) using immunofluorescence and the quantification of MMP-1 and IL-6 by ELISA assay. Moreover, the two formulations will be submitted to the evaluation of radical formation using electron paramagnetic resonance (EPR) spectroscopy. The collaboration with Prof. Dr. Monika Schäfer- Korting and Prof. Juergen Lademann group will contribute to the development of a successful reconstructed skin model and will increase the relevance of this project in order to produce more relevant publications with international groups and increase the impact of this research.