Involvement of the glial cells in the antinociceptive effect of Botulinum toxin type A

• Grant number: 18/13575-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 01, 2018
• End date: September 30, 2019
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Altair Antoninha Del Bel Cury
• Grantee: Victor Ricardo Manuel Muñoz Lora
• Supervisor: Zdravko Lackovic
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: University of Zagreb,
• Associated to the scholarship: 17/07741-7 - EFFECTS OF BONT-A PERIPHERAL-APPLICATION ON CENTRAL SENSITIZATION IN TMJ OF RATS, BP.DR

Abstract

The antinociceptive effect of botulinum toxin type A (BoNT-A) is, in part, due to the peripheral inhibition of pain mediators. On the other hand, authors indicate a central involvement of the neurotoxin, with a possible interaction with glial cells. Therefore, the aim of the study is to evaluate the possible interaction between BoNT-A and glial cells through the mark of expression of cleaved-SNAP-25, P2X7, and TRPV1 by immunofluorescence, in rats induced to persistent hypernociception. To achieve this goal, Wistar rats will be induced to persistent hypernociception in the Temporomandibular joint and then, treated with an intra-articular injection of BoNT-A (7U / kg). Behavioral tests will be applied using the rat grimace scale and animals will be euthanized to collect samples of the trigeminal ganglion and subnucleus caudalis to mark the expression of the cleaved-SNAP-25, P2X7, and TRPV1 by immunofluorescence. Data will be collected and the corresponding statistical analyzes will be applied.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LORA, V. R. M. MUNOZ; CURY, A. A. DEL BEL; JABBARI, B.; LACKOVIC, Z.. Botulinum Toxin Type A in Dental Medicine. JOURNAL OF DENTAL RESEARCH, v. 98, n. 13, p. 1450-1457, DEC 2019. (18/13575-5)

MANUEL MUNOZ-LORA, VICTOR RICARDO; OKROSA, ANA DUGONJIC; MATAK, IVICA; DEL BEL CURY, ALTAIR ANTONINHA; KALINICHEV, MIKHAIL; LACKOVIC, ZDRAVKO. Antinociceptive Actions of Botulinum Toxin A1 on Immunogenic Hypersensitivity in Temporomandibular Joint of Rats. TOXINS, v. 14, n. 3, p. 17-pg., 2022-03-01. (18/13575-5, 17/07741-7)