Antimicrobial and biocompatibility potentials of hexamethyldisiloxane films incorporated with chlorhexidine on titanium surface using a co-infected in vitro reconstituted gingival oral epithelium model

• Grant number: 17/21894-0
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: October 15, 2018
• End date: October 14, 2019
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Valentim Adelino Ricardo Barão
• Grantee: Adaias Oliveira Matos
• Supervisor: Richard L. Gregory
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Institution abroad: Indiana University-Purdue University Indianapolis, United States
• Associated to the scholarship: 16/06117-5 - Development of hexamethyldisiloxane films incorporated with chlorhexidine onto titanium surface: antimicrobial potential and cytotoxicity, BP.DR

Abstract

A stable connection between the titanium implant surface/abutment and periimplant soft tissues is an important approach for the long-term success of dental implant treatment. Thus, such surface should minimize the bacterial colonization while allowing a good adhesion of the oral tissues. Therefore, the objective of this study is to evaluate the antimicrobial and biocompatibility potentials of hexamethyldisiloxane films incorporated with and without (control) chlorhexidine (CHX) onto commercially pure titanium (cpTi) surface on a co-infected in vitro reconstituted oral epithelium model. Machined (M) surface will also be used as control. The development and characterization of such surface and its in vitro antimicrobial and biocompatibility potentials were tested in Brazil in the PhD scholarship project. However, we were not able to conduct co-infection model that represents a more realistic clinical scenario. Therefore, for the co-infection of an in vitro reconstituted oral epithelium assay, immunofluorescence imaging, metabolic activity, histology, cytotoxicity test (LDH release), viability test (MTT assay) and immunohistochemistry (Ki67 expression) will be conducted. Quantitative data will be statistically analyzed at a significant level of 5%. The number of samples for each assay will be determined by a pilot study.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ADAIAS OLIVEIRA MATOS; VALENTIM ADELINO RICARDO BARÃO; RICHARD LEE GREGORY. Nicotine is a potent extracellular polysaccharide inducer in Fusobacterium nucleatum biofilms. Braz. J. Oral Sci., v. 22, 2023-12-01. (17/21894-0)

MATOS, ADAIAS OLIVEIRA; DE ALMEIDA, AMANDA BANDEIRA; BELINE, THAMARA; TONON, CAROLINE C.; VIANA CASARIN, RENATO CORREA; WINDSOR, LESTER JACK; DUARTE, SIMONE; NOCITI JR, FRANCISCO HUMBERTO; RANGEL, ELIDIANE CIPRIANO; GREGORY, RICHARD L.; et al. Synthesis of multifunctional chlorhexidine-doped thin films for titanium-based implant materials. Materials Science & Engineering C-Materials for Biological Applications, v. 117, DEC 2020. (16/06117-5, 17/21894-0)

MATOS, ADAIAS OLIVEIRA; RANGEL, ELIDIANE CIPRIANO; RICARDO BARAO, VALENTIM ADELINO; GREGORY, RICHARD LEE. Antimicrobial behavior of titanium coating with chlorhexidine-doped thin film exposed to a biofilm supplemented with nicotine. DENTAL MATERIALS JOURNAL, v. 42, n. 2, p. 8-pg., 2023-04-01. (17/21894-0, 16/06117-5)