Objective: To detect and quantify colonizing of bacteria in the gingival crevicular fluid, to verify periodontal clinical parameters and marginal adaptation of the natural teeth submitted to aesthetic treatment with very thin laminate veneers without finish line and cervical margin placed within the gingival sulcus. Materials and Methods: 72 lithium disilicate laminate veneers, 0.2 mm thick at the cervical area, will be cemented onto unprepared maxillary anterior teeth. For all the treated and controls elements, the gingival crevicular fluid (GCF) will be collected prior to restoration cementation (baseline) and at times (t) after cementation: t0 - 7 days, t1 - 15 days, t2 - 30 days, t3 - 60 days, and t4 - 180 days. In each time, the GCF volume will be calculated the presence of the bacterias S. sanguis, S. mitis, P. intermedia and P. gingivalis will be quantified by use of PCR real- time. Clinically, will be evaluate the periodontal clinical parameters: plaque index (PI), bleeding on probing (BOP), probing depth (PD), clinical attachment level (CAL) and gingival resection (GR) and the evaluate of marginal adaptation in all times of study. Replicas of cervical area will be made with epoxy resin at (t0) 7, (t2) 30, and (t4) 180 days after cementation to verify the marginal adaptation and overcontour in the scanning electron microscope (SEM). Results: After the descriptive analysis of the data, the assumptions of normality and homogeneity will be verified, considering the application of ANOVA. If the assumptions are not met, the use of the Kruskall-Wallis test is foreseen. The significance level considered for difference between averages will be 5%.
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