The aims of this research are: a) To verify the microbial load (UFC/mL) and to quantify endotoxins (EU/mL) present in the dental pulp cavity of teeth with primary endodontic infection; b) To compare the antibacterial and endotoxin effectiveness of the biomechanical preparation with different final irrigation protocols: conventional irrigation, passive ultrasonic irrigation and continuous ultrasonic irrigation; c) To evaluate the microbial load (UFC/ml) and endotoxin levels after intracanal medication with Ca (OH) 2 + saline solution. 45 uniradicular teeth with presence of primary endodontic infection and periapical lesion will be selected for the study, being submitted to concomitant computed tomography. The channels will be prepared with a reciprocal system and irrigated with 2.5% NaOCl, followed by three final irrigation protocols: conventional irrigation (G1), passive ultrasonic irrigation (G2) and continuous ultrasonic irrigation (G3). All channels will be filled with Intracanal Medication with calcium hydroxide + physiological saline for 14 days and will be filled with gutta-percha cones and AH Plus cement. Three collections of the contents of the canal will be performed, soon after the coronary opening (1st Collection); after PBM performed the different protocols of final irrigation on activation with 2.5% NaOCl (2nd Collection) and after 14 days of the placement of Intracanal Medication (3rd Collection). The content of the collections will be evaluated by means of microbial culture and quantification of endotoxins by Limulus Amebocyte Lysate (LAL). The results will be submitted to statistical analysis.
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