Detection of toxin genes in Staphylococcus spp. isolated food manipulators

• Grant number: 18/12652-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2018
• End date: December 31, 2019
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Valéria Cataneli Pereira
• Grantee: Giovana do Nascimento Pereira
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

The pilot kitchen is a department benefiting from the National School Meal Program where meals are prepared and then distributed to municipal schools. In addition to the nutritional aspects, hygienic-sanitary conditions are essential for the protection and promotion of student health, since the incidence of Foodborne Diseases has grown worldwide to be considered a worrying public health problem. Contamination by bacterial origin is one of the sources that contributes to the high incidence of food-borne diseases, and the intake of pre-formed staphylococcal enterotoxins in food is involved in the vast majority of cases. In addition, food handlers colonized by Staphylococcus producing superantigens and leukotoxins are subject to infections, such as skin. Thus, this work aims to identify Staphylococcus sp. isolated from professionals in a pilot kitchen, as well as molecular detection of the genes of classic staphylococcal enterotoxins, TSST-1 and Leucocidin Panton Valentine. Thus, samples of Staphylococcus isolated from the nasal cavities and nails of food handlers from three pilot kitchens will be analyzed. These bacterial isolates will be identified by biochemical and multiplex PCR assays. The classical enterotoxin genes A, B, C and D (seb, seb, sec and sed genes), tst and luk-PVL will be screened by the PCR technique. For the analysis of the obtained results the Test of X2 will be used. The level of significance for all tests will be set at p <0.05

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GIOVANA DO NASCIMENTO PEREIRA; RAFAEL DA SILVA ROSA; ANDRÉ APARECIDO DIAS; DIEGO JÚNIOR SANTOS GONÇALVES; AMANDA APARECIDA SERIBELLI; LUIZA PINHEIRO-HUBINGER; LIZZIANE KRETLI WINKELSTROTER ELLER; THAIS BATISTA DE CARVALHO; VALERIA CATANELI PEREIRA. Characterization of the virulence, agr typing and antimicrobial resistance profile of Staphylococcus aureus strains isolated from food handlers in Brazil. Brazilian Journal of Infectious Diseases, v. 26, n. 5, 2022-11-11. (18/00056-0, 18/12652-6)