Comparative toxicity analysis of trifluralin and tebuthiuron herbicides and its commercial formulations: effects on embryo-larval developmental of Zebrafish (Danio rerio)

• Grant number: 18/13249-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2018
• End date: October 31, 2021
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Danielle Palma de Oliveira
• Grantee: Andréia Ávila Soares de Oliveira
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies, AP.TEM

Abstract

Trifluralin (TFL) and Tebuthiuron (TBR) herbicides occurrence in environment have been reported by several studies. However, data on its toxicity in non-target organisms are not well stablished and may be associated with its commercial formulations. Since previous results from our research group has demonstrated TFL toxicity potential only at high concentrations, this research project aims to apply sensitive tools either to identify and compare early effects at low concentrations between TFL, TBR and its commercial formulations in embryo-larval stages of Zebrafish (Danio rerio). For this purpose, lethal, sub-lethal and teratogenic effects will be evaluated by morphometric and functional analyses through DanioScopeTM software. Concerning to investigate possible neurotoxic effects, we purpose to determine acetylcholinesterase (AChE) activity followed by embryo activity monitoring analyses, which corresponds to early neurotoxicity biomarkers. Finally, as one of the most sensitive way to identify toxicants to developing organisms, we intend to analyze TFL and TBR effects on gene expression patterns related to neurotoxicity throughout embryonic development of D. rerio. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA, ANDREIA A. S.; BRIGANTE, TAMIRES A. V.; OLIVEIRA, DANIELLE P.. Tail Coiling Assay in Zebrafish (Danio rerio) Embryos: Stage of Development, Promising Positive Control Candidates, and Selection of an Appropriate Organic Solvent for Screening of Developmental Neurotoxicity (DNT). WATER, v. 13, n. 2, JAN 2021. (14/50945-4, 18/13249-0, 18/24298-2)