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Evaluation of the activity of circulating colony forming endothelial cells (ECFCs) in patients with spontaneous and induced Venous Thrombosis

Grant number: 18/15618-3
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2019
Effective date (End): April 30, 2022
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Joyce Maria Annichino-Bizzacchi
Grantee:Letícia Queiroz da Silva Justo
Host Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:16/14172-6 - Investigation of the pathophysiological aspects and novel therapeutic approaches for thromboembolic disorders, AP.TEM


Deep Vein Thrombosis (DVT) is defined as a pathological process resulting from alteration of vessel wall, blood flow, and/or composition blood flow, which leads to the formation of a thrombus, which can trigger other events. This process, although well described, remains indefinite in terms of several causes etiological factors that promote the disease. The vascular endothelium when actively responding to pathophysiological stimuli, can become vulnerable, and with the loss of its integrity, endothelial cells (CEPs) and colony-forming (CEFCs) to circulation. These changes have been correlated as possible markers for various diseases, as well as for cardiovascular events. Although the endothelium also play a key role in hemostasis, few endothelial cells in TVP. The use of oral anticoagulants during this evaluationis also a criticism of previous studies in the area. Therefore, the objective of this work will be to evaluate the proliferative activity and interface with CEFC hemostasis in patients with spontaneous and provoked DVT, compared withhealthy individuals. As a methodology, (1) we will identify and characterize the CEPs and CEFCs by flow cytometry. After this, (2) endothelial proliferative activity with the IncuCyte® incubator; (3) dosages of radicals free and nitric oxide; (5) evaluation of the pro-inflammatory, angiogenic and coagulation of these cells; (6) evaluation of intercellular interaction between CEFCs and platelets through a 3D co-culture assay by bioprinting and (7) identification of the released components, as well as adhesion molecules. (AU)

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
JUSTO, Letícia Queiroz da Silva. The role of endothelial colony-forming cells (ECFCs) in acute deep vein thrombosis patients. 2022. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.

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