Purification of milk proteins at pilot scale using membrane filtration and preparative chromatography

• Grant number: 18/24481-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2019
• End date: January 19, 2020
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Guilherme Miranda Tavares
• Grantee: Heice Silva Lopes Moraes
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 17/09214-4 - How do structural changes of dairy proteins affect the functionality of bioactive compounds in complex food matrices?, AP.JP

Abstract

The purification of high value-added macromolecules from food matrices represents a great opportunity to diversify activities of food industries. Despite the great accumulated knowledge about milk proteins, their large-scale purification usually does not preserve their native structure, which is crucial for their techno-functionalities. Thus, the objective of the present work is to develop protocols for the purification of globular milk proteins preserving their native structure through the association of tangential filtration techniques and preparative liquid chromatography.