High throughput biocompatible SPME coatings for metabolomic analyses

• Grant number: 18/25729-7
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): July 01, 2019
• Effective date (End): December 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry
• Principal Investigator: Fabio Augusto
• Grantee: Joao Raul Belinato de Souza
• Supervisor abroad: Janusz Pawliszyn
• Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Local de pesquisa : University of Waterloo, Canada
• Associated to the scholarship: 16/20547-2 - Characterization of the metabolome of anti-phytopatogen microorganisms by multidimensional gas chromatography and simple / tandem mass spectrometry (GC×GC-QMS and GC×GC-MS/MS), BP.DR

Abstract

This doctorate research internship abroad (BEPE-DR) project is a part of the National Institute of Bioanalytical Science and Technology (INCTBio) and FAPESP doctorate project (Proc. 2016 / 20547-2). This project aims to study high throughput Thin-Film SPME (TF-SPME) devices for global metabolic profiling of biological samples. These systems will be used as model for targeted and untargeted metabolomics studies. TF-SPME devices will be prepared and characterized according to standard procedures found in literature. TF-SPME will be initially used to explore non-volatile metabolome of biological samples (untargeted and targeted) using LC-MS systems. After that, volatile metabolome from biological samples' systems will be evaluated using GCxGC-TOFMS platforms. All experiments will be done at the University of Waterloo, Canada, under supervision of Professor Janusz Pawliszyn.