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Evaluation of the viscosity and applicability of different products marketed for the conditioning of enamel and dentin

Grant number: 18/20586-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2019
Effective date (End): December 31, 2019
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Maria Angela Pita Sobral Fernandes
Grantee:Vitoria Rocha Bico
Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Phosphoric acid has been used in dentistry since 1955 when it was first tested by Michael Buonocore. With the success of the technique, composite resin restorations presented better adhesion results, generating less infiltration (BUONOCORE, 1995).Realizing the lack of information on the physicochemical properties of phosphoric acid, as the viscosity suitable for clinical application, the present study proposes in its first step to select some acids commercialized determining its viscosity. In the second step five acids will be selected according to the viscosity to analyze its applicability. In the third step, the five acids of the second stage will be analyzed by scanning electron microscopy. In the first stage will be selected 10 different trademarks of phosphoric acid. A "pool" of each acid will be obtained until 10 ml is obtained. The viscosity of the acids will be measured 3 times at 24ºC and 37ºC in a Viscometer. Another measurement will be done after 24 hours at room temperature. In the second stage 5 of the 10 acids of the first step will be selected, two being more viscous, two less viscous and one intermediate. These acids will pass through the assessment of clinical applicability. Clinically active dental surgeons will apply it to a natural tooth to understand how viscosity interferes with practice. In the third stage 10 human natural molars donated by FOUSP Biobank, will be prepared and conditioned with the 5 acids of the second phase for SEM analysis, qualitatively illustrating the conditioning variance between the acids analyzed. To compare the results, ANOVA and Tukey test (p> 0.05).

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