Application of nanoemulsions with essential oils as natural coatings for application in post-harvesting strawberry conservation

• Grant number: 18/24612-9
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2019
• End date: February 28, 2022
• Field of knowledge: Agronomical Sciences - Agricultural Engineering - Process Engineering of Agricultural Products
• Principal Investigator: Marcos David Ferreira
• Grantee: Josemar Gonçalves de Oliveira Filho
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated scholarship(s): 19/15363-8 - Essential oils as natural fungicides on postharvest quality strawberry fruit: evaluation of the application in coatings and slow release microencapsulated pouches, BE.EP.DR

Abstract

Edible coatings, including active substances, can be used as an alternative to preserve fruits and vegetables during post-harvest. Essential oils (EOs) exhibit antimicrobial activity and by this characteristic have been proposed as natural fungicides.The use of nanoemulsions, as a method to disperse bioactive agents, is emerging as a potential tool for designing new coatings with optimum functionality. In this context, the objective is to evaluate the potential of EOs as natural fungicides and their presence in nanostructured coatings for application in quality maintenance and post-harvest conservation of strawberries, as a proposal to use natural agents of vegetable origin as active materials for coating fruits. The EOs of the species Green Mint (Mentha spicata), Peppermint (Mentha x piperita), Palmarosa (Cymbopogon martinii) and Pau-rosa (Aniba rosaeodora) will be obtained the chemical compositions will be evaluated by CG-SM and CG-FID, and the antifungal activity, in in vitro analyzes for the main post-harvest strawberry fungi. Compound coatings will be produced using hydroxypropylmethylcellulose (HPMC), nanoemulsified carnauba wax and cellulose nanocrystals and will be incorporated with EO which exhibits the highest antifungal activity in the in vitro tests. The coating and EO combinations which exhibit the best barrier properties and antifungal activity will be tested in vivo. Initially, the effect of EO coatings on the control of Rhizopus stolonifer and Botrytis cinerea on infected strawberries will be studied in order to evaluate the effect of coatings on the control of post-harvest diseases. The strawberries were then coated and evaluated during storage (0, 2, 4, 6, 8, 10 and 12 days) for soluble solids content, pH, titratable acidity, maturation index, concentration of anthocyanins, total phenols, antioxidant activity, total fungi count, aerobic mesophilic bacteria, coliform bacteria, visual fungal deterioration, strawberry volatile profile by GC-MS, and sensorial acceptance using a nine-point scale.

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; ALBIERO, BEATRIZ REGINA; CIPRIANO, LAVINIA; DE OLIVEIRA NOBRE BEZERRA, CARMEN CRIS; OLDONI, FERNANDA CAMPOS ALENCAR; EGEA, MARIANA BURANELO; DE AZEREDO, HENRIETTE MONTEIRO CORDEIRO; FERREIRA, MARCOS DAVID. Arrowroot starch-based films incorporated with a carnauba wax nanoemulsion, cellulose nanocrystals, and essential oils: a new functional material for food packaging applications. Cellulose, v. 28, n. 10, p. 6499-6511, JUL 2021. (18/24612-9)

SILVA, GULHERME DA CRUZ; DE OLIVEIRA FILHO, JOSEMAR GOLCALVES; MORSELLI RIBEIRO, MARILENE DE MORI; OLIVEIRA DE SOUZA, CLOVIS WESLEY; FERREIRA, MARCOS DAVID. Antibacterial Activity of Nanoemulsions Based on Essential Oils Compounds Against Species of Xanthomonas that Cause Citrus Canker. BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY, v. 12, n. 2, p. 1835-1846, APR 15 2022. (18/24612-9)

EGEA, MARIANA BURANELO; BERTOLO, MIRELLA ROMANELLI VICENTE; OLIVEIRA FILHO, JOSEMAR GONCALVES DE; LEMES, AILTON CESAR. A Narrative Review of the Current Knowledge on Fruit Active Aroma Using Gas Chromatography-Olfactometry (GC-O) Analysis. Molecules, v. 26, n. 17, SEP 2021. (19/18748-8, 18/24612-9)

DE OLIVEIRA FILHO, JOSEMAR GONSALVES; ALBIERO, BEATRIZ REGINA; CALISTO, ITALO HENRIQUE; BERTOLO, MIRELLA ROMANELLI VICENTE; OLDONI, FERNANDA CAMPOS ALENCAR; EGEA, MARIANA BURANELO; BOGUSZ JUNIOR, STANISLAU BOGUSZ; DE AZEREDO, HENRIETTE MONTEIRO CORDEIRO DE; FERREIRA, MARCOS DAVID. Bio-nanocomposite edible coatings based on arrowroot starch/cellulose nanocrystals/carnauba wax nanoemulsion containing essential oils to preserve quality and improve shelf life of strawberry. International Journal of Biological Macromolecules, v. 219, p. 12-pg., 2022-08-12. (18/24612-9, 19/18748-8)

OLDONI, FERNANDA C. A.; BERNARDO, MARCELA P.; FILHO, JOSEMAR G. OLIVEIRA; DE AGUIAR, ALINE C.; MOREIRA, FRANCYS K. V.; MATTOSO, LUIZ H. C.; COLNAGO, LUIZ A.; FERREIRA, MARCOS D.. Valorization of mangoes with internal breakdown through the production of edible films by continuous solution casting. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 145, JUN 2021. (18/07860-9, 19/13656-8, 18/24612-9)

OLIVEIRA FILHO, JOSEMAR GONCALVES; SILVA, GUILHERME DA CRUZ; EGEA, MARIANA BURANELO; CORDEIRO DE AZEREDO, HENRIETTE MONTEIRO; FERREIRA, MARCOS DAVID. Essential Oils as Natural Fungicides to Control Rhizopus stolonifer-Induced Spoiled of Strawberries. BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY, v. 11, n. 5, p. 13244-13251, OCT 15 2021. (18/24612-9)

DE OLIVEIRA FILHO, JOSEMAR GONSALVES; DE OLIVEIRA NOBRE BEZERRA, CARMEN CRIS; ALBIERO, BEATRIZ REGINA; ALENCAR OLDONI, FERNANDA CAMPOS; MIRANDA, MARCELA; EGEA, MARIANA BURANELO; CORDEIRO DE AZEREDO, HENRIETTE MONTEIRO; FERREIRA, MARCOS DAVID. New approach in the development of edible films: The use of carnauba wax micro- or nanoemulsions in arrowroot starch-based films. FOOD PACKAGING AND SHELF LIFE, v. 26, DEC 2020. (18/24612-9)

BERTOLO, MIRELLA ROMANELLI VICENTE; DIAS, LUCAS DANILO; FILHO, JOSEMAR GONCALVES DE OLIVEIRA; ALVES, FERNANDA; MARANGON, CRISIANE APARECIDA; AMARO MARTINS, VIRGINIA DA CONCEICA; FERREIRA, MARCOS DAVID; BAGNATO, VANDERLEI SALVADOR; PLEPIS, ANA MARIA DE GUZZI; BOGUSTZ JUNIOR, STANISLAU. Central composite design optimization of active and physical properties of food packaging films based on chitosan/gelatin/pomegranate peel extract. FOOD PACKAGING AND SHELF LIFE, v. 34, p. 11-pg., 2022-11-14. (13/07276-1, 14/50857-8, 19/13569-8, 18/22214-6, 18/24612-9, 19/22799-7, 19/18748-8)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; SILVA, GUILHERME DA CRUZ; GOMES, MARIANA DE PAULA; SOUSA, TAINARA LEAL DE; FERREIRA, MARCOS DAVID; EGEA, MARIANA BURANELO. External application of RNA interference (RNAi): an innovative tool for controlling fungi during food storage. CURRENT OPINION IN FOOD SCIENCE, v. 47, p. 6-pg., 2022-10-01. (18/24612-9)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; VICENTE BERTOLO, MIRELLA ROMANELLI; VIGILATO RODRIGUES, MURILO ALISON; SILVA, GUILHERME DA CRUZ; NAVARRO DE MENDONCA, GIOVANA MARIA; JUNIOR, STANISLAU BOGUSZ; FERREIRA, MARCOS DAVID; EGEA, MARIANA BURANELO. Recent advances in the development of smart, active, and bioactive biodegradable biopolymer-based films containing betalains. Food Chemistry, v. 390, p. 13-pg., 2022-05-13. (18/24612-9, 19/18748-8)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; SILVA, GUILHERME DA CRUZ; ALENCAR OLDONI, FERNANDA CAMPOS; MIRANDA, MARCELA; FLORENCIO, CAMILA; DUARTE DE OLIVEIRA, RAISSA MORET; GOMES, MARIANA DE PAULA; FERREIRA, MARCOS DAVID. Edible Coating Based on Carnauba Wax Nanoemulsion and Cymbopogon martinii Essential Oil on Papaya Postharvest Preservation. COATINGS, v. 12, n. 11, p. 9-pg., 2022-11-01. (18/10657-0, 18/24612-9, 16/23419-5)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; DA CRUZ SILVA, GUILHERME; DE AGUIAR, ALINE CRISTINA; CIPRIANO, LAVINIA; DE AZEREDO, HENRIETTE MONTEIRO CORDEIRO; BOGUSZ JUNIOR, STANISLAU; FERREIRA, MARCOS DAVID. Chemical composition and antifungal activity of essential oils and their combinations against Botrytis cinerea in strawberries. JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, v. 15, n. 2, p. 1815-1825, APR 2021. (18/24612-9)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; VICENTE BERTOLO, MIRELLA ROMANELLI; VIGILATO RODRIGUES, MURILO ALISON; MARANGON, CRISIANE APARECIDA; SILVA, GUILHERME DA CRUZ; ALENCAR ODONI, FERNANDA CAMPOS; EGEA, MARIANA BURANELO. Curcumin: A multifunctional molecule for the development of smart and active biodegradable polymer-based films. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 118, n. B, p. 840-849, DEC 2021. (19/22799-7, 18/22214-6, 18/24612-9)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; VICENTE BERTOLO, MIRELLA ROMANELLI; BRITO, SABRINA DA COSTA; DONIZETTE MALAFATTI, JOAO OTAVIO; BERTAZZO, GIOVANA BRAIT; COLACIQUE, MATHEUS NUNES; PARIS, ELAINE CRISTINA; BOGUSZ JUNIOR, STANISLAU; FERREIRA, MARCOS DAVID. Recent Advances in the Application of Nanotechnology to Reduce Fruit and Vegetable Losses During Post-Harvest. Brazilian Journal of Physics, v. 52, n. 4, p. 11-pg., 2022-08-01. (19/18748-8, 18/24612-9)

DE OLIVEIRA FILHO, JOSEMAR GONCALVES; MIRANDA, MARCELA; FERREIRA, MARCOS DAVID; PLOTTO, ANNE. Nanoemulsions as Edible Coatings: A Potential Strategy for Fresh Fruits and Vegetables Preservation. FOODS, v. 10, n. 10, OCT 2021. (18/24612-9, 18/10657-0, 16/23419-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA FILHO, Josemar Gonçalves de. Plant-based nanoemulsions with essential oils as edible coatings: A novel approach for strawberry preservation. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.