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Evaluation of wound healing in mice treated with alcoholic extract from Dipterix alata Vogel (Leguminosae)

Grant number: 18/25061-6
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Bruno Watanabe Minto
Grantee:Marcelo Candido Portilho Gouveia
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Aiming to preserve the flora and fauna of the Brazilian biome called "Cerrado", researchers are looking into medicinal properties of native plants. The species Dipteryx alata vogel (Baru) is a native plant of Cerrado, and may have a plethora of nutritional and pharmacological properties.The main goal of this study is to evaluate through histological (Hematoxylin/Eosin and Psicrosirius) and immunohistochemistry (Antibodies CD31 and Ae1/Ae3) the influence of the above-mentioned species in the wound healing process of standardized dermoepidemal wounds. Wounds are going to be made with a surgical punch in the dorsal thoracic region of mice (lineage C57BL6) and the wound size is going to be 0,9mm. There will be a total of 6 groups of study: CM (n= 9 males) and CF (9 females), this group is going to be the control one and will only be administered the vehicle without the active ingredient; TCM (n= 9 males) TCF (n= 9 females) will be administered an 10% extract of the outside layer (peel); TSM (n= 9 males) TSF (n= 9 females) will be administered an 10% extract of seed. All animals will receive the above-mentioned protocols according to each group twice a day until complete wound healing, around 20 days of treatment. The wounds will not be bandaged and the animal will be placed in sterile wood trims (changed every 3 days) in order to avoid contamination. The data obtained will be analyzed through Kruskal-Wallis method, Dunn multiple comparison test; Turkey test, SAS and GraphPad Prism (software programs) in order to evaluate the effect of Dipteryx alata vogel in the wound healing.

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