Protective effect of TiF4/NaF and chitosan solutions on the development of early enamel erosion and erosive enamel wear in vitro

• Grant number: 19/00778-8
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: July 01, 2019
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Ana Carolina Magalhães
• Grantee: Larissa Rodrigues Pereira Santi
• Supervisor: Thiago Saads Carvalho
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: University of Bern, Switzerland
• Associated to the scholarship: 16/24538-8 - Effect of experimental mouthrinses containing NaF and TiF4 on dental erosion and abrasion in situ, BP.IC

Abstract

This study will analyze the protective effect of experimental solutions containing TiF4/NaF and chitosan (at different viscosities) on early enamel erosion (by reflectometry) and erosive enamel wear (by profilometry) in vitro. For this, bovine enamel samples (n=15) will be distributed to the following treatment groups: (1) commercial solution with SnCl2/NaF (800 ppm Sn+2, 500 ppm F, pH 4.5, Elmex®, GABA Int. AG, Switzerland, positive control); (2) 0.042% NaF and 0.049% TiF4 (NaF: 190 ppm F; TiF4: 190 ppm Ti4+ and 300 ppm F); (3) similar to 2 but with addition of 0.5% chitosan (Ch 50 mPas), (4) similar to 2 but with addition of 0.5% chitosan (Ch 2000 mPas), (5) no treatment (negative control), (6) 0.5% chitosan (Ch 50 mPas) and (7) 0.5% chitosan (Ch 2000 mPas). The samples will be submitted to pH cycles (0.1% citric acid 4x90s/day interposed by artificial saliva) and daily treatment application (after the last erosive challenge, 1x30s/day) for seven days. After the 1stday, the surface reflection intensity changes will be measured. After 7 days, the erosive enamel wear will be quantified using a contact profilometer. The data will be statistically compared (p < 0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUZA, BEATRIZ MARTINES; PEREIRA SANTI, LARISSA RODRIGUES; JOAO-SOUZA, SAMIRA HELENA; CARVALHO, THIAGO SAADS; MAGALHAES, ANA CAROLINA. Effect of titanium tetrafluoride/sodium fluoride solutions containing chitosan at different viscosities on the protection of enamel erosion in vitro. ARCHIVES OF ORAL BIOLOGY, v. 120, DEC 2020. (16/24538-8, 19/00778-8)