Endometrial expression of inflammatory cytokines and proteomics of endometrial fluid of susceptible mares to persistent breeding-induced endometritis treated with platelet-rich plasma

• Grant number: 19/05017-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: May 18, 2019
• End date: December 30, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Marco Antonio Alvarenga
• Grantee: Lorenzo Garrido Teixeira Martini Segabinazzi
• Supervisor: Igor Frederico Canisso
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Institution abroad: University of Illinois at Urbana-Champaign, United States
• Associated to the scholarship: 17/13883-9 - ENDOMETRIAL EXPRESSION OF INFLAMMATORY INTERLEUKINS AND PROTEOMICS OF ENDOMETRIAL FLUID OF MARES SUSCEPTIBLE TO PERSISTENT BREEDING-INDUCED ENDOMETRITIS TREATED WITH PLASMA RICH PLASMA, BP.DR

Abstract

In the horse market the offspring of proven mares are more valued. However, this usually happens when mares already have advanced age and progeny proven in the competitions. This encourages the maintenance of older in breeding program despite low reproductive efficiency. Older mares are more susceptible to persistent breeding-induced endometritis (PBIE). This condition is the main cause of subfertility in mares and is the third most frequent disease affecting horses. Studies using immunomodulatory agents such as platelet-rich-plasma (PRP) have shown promising results in reducing the inflammation and promoting fertility of these animals. The PRP is gaining popularity in veterinary medicine for its mitogenic, chemotactic, neovasculazation and anti-inflammatory action, in the treatment of a wide range of diseases in tendins, joints, ligaments, and tegument. In this context, the present study aims to evaluate the endometrial expression of pro- and anti-inflammatory cytokines of mares treated with PRP, and proteome of the endometrial fluid of these animals. Our recent study, has shown the clinical benefits of PRP in the treatment of susceptible mares to PBIE by modulating the uterine inflammatory response by decreasing the number of neutrophils and COX-2 expression in the endometrium of these animals. However, further studies of this therapy in the treatment of mares susceptible to PBIE are necessary to better understand the mechanism of action of these agents in the mare endometrium.