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Phytochemical study and cytotoxic evaluation of the hexanic fraction and isolated compounds from red propolis produced by Apis mellifera L.

Grant number: 19/01697-1
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2019
Effective date (End): August 31, 2021
Field of knowledge:Health Sciences - Pharmacy - Pharmacognosy
Principal Investigator:Jairo Kenupp Bastos
Grantee:Matheus Hikaru Tanimoto
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector, AP.TEM


Propolis is a natural resinous substance produced by bees, including the species Apis mellifera L., from parts of plants, or exudates, which are mixed with wax and salivary secretion. Its composition and, consequently, its biological activities depend on several factors, such as geographic origin, seasonality, sun light, altitude and availability of food during the production of propolis. There are many biological properties reported for propolis. Among the different types of Brazilian propolis, there is the need for further studies on red propolis from Dalbergia ecastophyllum (L.) Taub., mainly on its hexanic extract and isolated compounds, which display antiproliferative action. Considering the economic and pharmacological potentials of red propolis we are proposing the phytochemical study of its hydroalcoholic extract, followed by partition with solvents of increasing polarity and isolation of the major constituents of the hexanic fraction using different chromatographic modalities, such as: open-column chromatography, chromatotron, and preparative HPLC, among others. The obtained fractions will be analyzed by using analytical thin-layer chromatography, and the isolated compounds will be identified by spectroscopic and spectrometric means, mainly through nuclear magnetic resonance of hydrogen and/or carbon and high resolution mass spectrometry. The propolis extract, its hexanic fration and its isolated compounds will be evaluated for in vitro citotoxic activity against MCF-7 and SK-BR-3 breast cancer cell lines

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CCANA-CCAPATINTA, GARI VIDAL; ALDANA MEJIA, JENNYFER ANDREA; TANIMOTO, MATHEUS HIKARU; GROPPO, MILTON; ANDRADE SARMENTO DE CARVALHO, JEAN CARLOS; BASTOS, JAIRO KENUPP. Dalbergia ecastaphyllum (L.) Taub. and Symphonia globulifera L.f.: The Botanical Sources of Isoflavonoids and Benzophenones in Brazilian Red Propolis. Molecules, v. 25, n. 9 MAY 2020. Web of Science Citations: 0.

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