Proteomic analysis of periodontal tissues with mechanical orthodontic force on health or obesity - in vivo and in vitro study.

• Grant number: 18/25811-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2019
• End date: August 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Joni Augusto Cirelli
• Grantee: Camila Chierici Marcantonio
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The aim of this research project is to evaluate in vivo and in vitro the effect of obesity on periodontal tissue remodeling induced by mechanical orthodontic force. For the in vivo study, the samples collected in the student Master's research (2016/00732- 0) will be used. Proteomics analysis of the animals periodontal ligament induced by orthodontic movement and submitted or not to obesity induced will be microdissected through laser capture microdissection (LCM). These samples will be used and to liquid chromatrography-high resolution mass spectrometry (LC-MS/MS) analysis. Immunohistochemical analysis will be used to confirm the detection and localization of the six more relevant proteins previous found by proteomics analysis. Furthermore, the gene expression of these proteins will be evaluated on the animals gingival tissue by RT-qPCR. For the in vitro study, a human immortalized periodontal ligament-derived cell line will be use. These cells will be exposed to a static tensile stain (STS) on a high magnitude (STSH), with the association or not of Adiponectin and NAMPT stimulation. The gene expression of six more relevant proteins previous found on in vivo study will be evaluated by RT-qPCR. These proteins will be confirmed by ELISA and intracellular signaling and nuclear translocations induced will be analyzed by immunofluorescence. All data will be evaluated, and then subjected to normality test. Then, appropriate statistical tests for comparisons between groups will be used. For all tests, a 5% significance level will be adopted.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARCANTONIO, CAMILA CHIERICI; LOPES, MARIA EDUARDA SCORDAMAIA; MOFATTO, LUCIANA SOUTO; SALMON, CRISTIANE RIBEIRO; DESCHNER, JAMES; NOCITI-JUNIOR, FRANCISCO HUMBERTO; CIRELLI, JONI AUGUSTO; NOGUEIRA, ANDRESSA VILAS BOAS. Obesity affects the proteome profile of periodontal ligament submitted to mechanical forces induced by orthodontic tooth movement in rats. JOURNAL OF PROTEOMICS, v. 263, p. 10-pg., 2022-07-15. (16/00732-0, 14/20715-7, 17/07137-2, 18/25811-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARCANTONIO, Camila Chierici. Avaliação dos tecidos periodontais de ratos submetidos a força mecânica ortodôntica em condições de saúde ou doença sistêmica. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.