In vitro cellular reprogramming to pluripotency (iPS) in the swine model from non-transgenic and non-invasive methodology

• Grant number: 19/02811-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2019
• End date: December 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Fabiana Fernandes Bressan
• Grantee: Kaiana Recchia
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated research grant: 15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach, AP.JP

Abstract

Embryonic stem cells (ESC) are able to differentiate in the three embryonic leaflets due to their undifferentiated state, but their collection promotes on the embryo (blastocyst) infeasible and consequently faces ethical problems in the human model. However, with the possibility of inducing a differentiated cell to an undifferentiated state through the expression of embryonic pluripotency factors, it was possible to produce induced pluripotent cells (iPS), being interesting for regenerative medicine and cell therapy due to its high potential in both veterinary medicine as well as human. However, with the possibility of inducing a differentiated cell to an undifferentiated state through the expression of embryonic pluripotency factors, it was possible to produce induced pluripotent cells (iPS), being interesting for regenerative medicine and cell therapy due to its high potential in both veterinary medicine as well as human. O estudo do modelo suíno torna-se mais interessante devido a sua maior compatibilidade e viabilidade com o modelo humano, sendo semelhanças fisiológicas, morfológicas, sistema imunológico, entre outros. Consequently to the possibility of induction of pluripotent cells through the STEMCCA vector and of episomal vectors, in this study we intend to generate transgenic and non-transgenic iPS cells, respectively, from three different tissues and collect, being: fibroblasts from skin biopsy cells blood mononuclear cells from peripheral blood collection and progenitor cells from collecting urine from the swine model. To perform the characterization of the iPS cells obtained from the different tissues and collections by the alkaline phosphatase test, morphology and in vitro differentiation, for later analysis of the results and comparison of the efficiency of the cells and evaluating the possibility of working with genetic engineering with focus in regenerative and translational medicine. From the results obtained in conjunction with other results of the projects developed in the laboratory, these will be used as a basis for future differentiation experiment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RECCHIA, KAIANA; MACHADO, LUCAS SIMOES; BOTIGELLI, RAMON CESAR; PIERI, NAIRA CAROLINE GODOY; BARBOSA, GABRIELA; DE CASTRO, RAQUEL VASCONCELOS GUIMARAES; MARQUES, MARIANA GROKE; PESSOA, LAIS VICARI DE FIGUEIREDO; FANTINATO NETO, PAULO; MEIRELLES, FLAVIO VIEIRA; et al. In vitro induced pluripotency from urine-derived cells in porcine. WORLD JOURNAL OF STEM CELLS, v. 14, n. 3, p. 14-pg., 2022-03-26. (15/26818-5, 13/08135-2, 19/02811-2)

RECCHIA, KAIANA; PESSOA, LAIS VICARI DE FIGUEIREDO; PIERI, NAIRA CAROLINE GODOY; PIRES, PEDRO RATTO LISBOA; BRESSAN, FABIANA FERNANDES. Influence of Cell Type in In Vitro Induced Reprogramming in Cattle. LIFE-BASEL, v. 12, n. 8, p. 10-pg., 2022-08-01. (19/02811-2, 13/08135-2, 15/26818-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

RECCHIA, Kaiana. In vitro cellular reprogramming into pluripotency (generation of iPS cells) in the porcine model using non-transgenic and non-invasive methodology. 2020. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.