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Cellulases production in biorefineries by solid-state fermentation in packed-bed bioreactors

Grant number: 18/16689-1
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2019
Effective date (End): March 31, 2021
Field of knowledge:Engineering - Chemical Engineering
Principal Investigator:Fernanda Perpétua Casciatori
Grantee:Natalia Alvarez Rodrigues
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Associated research grant:16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales, AP.BIOEN.TEM

Abstract

At a time when the technological development of a clean and renewable energy matrix is strategic for Brazil, the concept of biorefinery emerges as that of an industry focused on the production of biofuels, with emphasis on bioethanol, in parallel to the production of high value biomolecules aggregate. With focus on the synthesis of second generation ethanol by biochemical route, the production of cellulolytic enzymes inside the industry (in-house) can guarantee self-sufficiency of the unit. For this purpose, solid state fermentation (SSF) is a viable alternative for obtaining these enzymes and other molecules of interest of the biorefinery, either as internal inputs or final products, using as raw materials sub or co-products of the biorefinery itself .In view of the above, this MSc project is focused on the construction and operation of a packed bed bioreactor for the production of cellulases by SSF. Experimental tests will be carried out in a modular packed bed bioreactor, which will be operated in batch mode and in pseudo-continuous mode, unprecedented in an experimental setting. Instrumentation of the equipment for monitoring the process is also planned. At the end of the project, it is expected to have a viable bio-process of obtaining cellulases using SSF bioreactors in a packed bed of practical and economic operation.The project is part of the Biochemical Engineering research area of the Chemical Engineering Department (DEQ) and will assist the Regular Research Project coordinated by the supervisor (Proc. FAPESP 2018/00996-2). It is also related to the Thematic Project (Proc. FAPESP 2016/10636-8) in progress at DEQ/UFSCar.

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