Genome assembly for identification of antibiotic resistance genes in clinical isolates of the Hospital das Clínicas de Ribeirão Preto

• Grant number: 19/06672-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2019
• End date: May 31, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Rafael Silva Rocha
• Grantee: Murilo Henrique Anzolini Cassiano
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 12/22921-8 - Synthetic biology approaches for deciphering the logic of signal integration in complex bacterial promoters, AP.JP

Abstract

The problem of antibiotic resistance has become a threat to global health. Increasingly, we are creating an atmosphere of selective pressure that has never been seen before by bacteria, due to either the use or misuse of antibiotics or any environmental contamination due to anthropological action. It is known that genes that confer resistance to antibiotics are both in pathogens and in environmental bacteria, evolving and spreading in the environment. Understanding how this process occurs can help in the epidemiological control of this growing threat. For this understanding, exploring the complex genetic content of pathogens, through sequencing and application of bioinformatics tools, is necessary and may open new possibilities of information, which can also be applied to the clinical context. The aim of this work is to assemble and analyze clinical isolated bacterial genomes, with different profiles of resistance to antibiotics, that were isolated at Hospital das Clínicas, Medical School of Ribeirão Preto, to identify genes and mechanisms of resistance present in the hospital environment.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORELLI, TIAGO CABRAL; LOVATE, GABRIEL LENCIONI; SCARANELLO, ANA FLAVIA TONELLI; RIBEIRO, LUCAS FERREIRA; ZARAMELA, LIVIA; PEREIRA-DOS-SANTOS, FELIPE MARCELO; SILVA-ROCHA, RAFAEL; GUAZZARONI, MARIA-EUGENIA. Combining Functional Genomics and Whole-Genome Sequencing to Detect Antibiotic Resistance Genes in Bacterial Strains Co-Occurring Simultaneously in a Brazilian Hospital. ANTIBIOTICS-BASEL, v. 10, n. 4, APR 2021. (19/00390-0, 15/04309-1, 19/06672-7, 16/18827-7, 19/15675-0, 18/18158-3)

ANZOLINI CASSIANO, MURILO HENRIQUE; SILVA-ROCHA, RAFAEL. Benchmarking Bacterial Promoter Prediction Tools: Potentialities and Limitations. MSYSTEMS, v. 5, n. 4, p. 16-pg., 2020-07-01. (19/15675-0, 19/06672-7, 12/22921-8)