THE PROTECTOR EFFECT OF HIDRATATION IN CONTRAST INDUCED NEPHROPATHY IN DIABETIC RATS

• Grant number: 19/07968-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: November 30, 2020
• Field of knowledge: Health Sciences - Nursing - Medical-Surgical Nursing
• Principal Investigator: Cassiane Dezoti da Fonseca
• Grantee: Dayse Santana Santos
• Host Institution: Escola Paulista de Enfermagem (EPE). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Introduction: Acute kidney injury (AKI) is characterized by increased serum creatinine and urinary output in 48 hours after clinical, surgical and infectious events. Among the causes of AKI is contrast-induced nephropathy (CIN), which has diabetes mellitus as a predisposing factor. Objective: To verify the effect of saline hydration on renal function, renal hemodynamics and on the oxidative profile of CIN in diabetic rats. Methods: 28 Wistar rats, male and adult, weighing between 250-290g will be used. The animals will be randomized into the following experimental groups: a) Citrate: animals that will receive the citrate buffer at pH 4.2, intravenous (i.v.); b) Diabetes (DM): animals receiving 65 mg / kg of streptozotocin, i.v., diluted in 0.1 M citrate buffer at pH 4.2 on the 1st day of protocol and will continue to be followed up until the 28th day of the experimental protocol; c) Diabetes + Iodinated contrast (DM + CI): DM animals receiving 6 ml / kg IC, intraperitoneal (i.p.), single dose on the 26th day of the experimental protocol; d) Diabetes + Iodinated Contrast + Saline Hydration (DM + CI + H): DM + CI animals that will receive 12ml / kg of NaCl 0.9% i.p. on the 23rd day, for five days. Physiological parameters such as weight, feed intake and water, glycemia and kidney weight and animal weight ratio will be evaluated; renal function (clearance of inulin and creatinine); renal hemodynamics and oxidative profile (urinary peroxides and TBARS, renal tissue thiols and urinary nitric oxide). The results will be expressed as mean ± standard deviation. The variance between the groups will be analyzed using the One Way ANOVA test, followed by the Newman-Keuls multiple-comparator post-test of the Graph-Pad Prism version-3 for Windows® statistical program. Values of p <0.05 will be considered significant. Expected results: Based on data analysis, the present investigation may contribute to elucidate whether pretreatment with saline hydration represents a therapeutic alternative in the prevention of CIN.