Development of yolk sac organoids from mammals

• Grant number: 18/24273-0
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): August 01, 2019
• Effective date (End): January 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal researcher: Carlos Eduardo Ambrósio
• Grantee: Vitória Mattos Pereira
• Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil

Abstract

The importance of hematopoietic, immunologic, metabolic and nutritional functions of yolk sac (YS) is already known during early embryonic development. But the knowledge about the mechanism of transport of YS and its dysfunction in yearly miscarriage remains unclear. To solve these problems, the organoid culture seems to be a good choice, due to its three-dimensional (3D) model that preserves the mean functions and genetic stability of the tissue in vitro. Further, this culture can be used for disease modelling and toxicology/pharmaceutical studies. The bovine, swine and canine models were chosen because of the different types of placentation, as sinepteliochorial, epitheliochorial and hemochorial respectively. In order to compare the characteristics of future structures formed and their transport functions during the placentation process. For the development of the yolk sac organoid, we choose the matrigel (Corning) technique and different culture media protocols to test growth factors supplementation. To characterize the formed structures we will use the techniques of RT-PCR, immunohistochemistry and electron microscopy.